A searching of novel biomarker in digestive organ cancer serum and the establishment of a practical diagnostic method using unique high-yield peptide-extraction method.

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790410
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Chiba University
• Principal Investigator: SATOH Mamoru Chiba University, 医学部・附属病院, 助教 (20401002)
• Co-Investigator(Renkei-kenkyūsha): NOMURA Fumio 千葉大学, 大学院・医学研究院, 教授 (80164739) ; TOMONAGA Takeshi 独立行政法人医薬基盤研究所, 研究員 (80227644) ; KODERA Yoshio 北里大学, 理学部, 准教授 (60265733) ; SOGAWA Kazuyuki 千葉大学, 医学部附属病院, 助教 (50436440) ; KAWASHIMA Yusuke 北里大学, 理学部, ボストドク
• Project Period (FY): 2008 – 2009
• Keywords: 臨床検査医学 / プロテオミクス

Research Abstract

健常人15例、食道癌術前患者13例、術後患者11例の血清の解析を行った結果、食道癌患者血清で増加しているピークを20ピーク検出できた。これらのピーク強度に差の認められたペプチドの1司定を行った。しかしながら、同定できたピークは1ピークとなっている。この原因としては、さまざまな翻訳後修飾(細胞内で起こるもの以外を含む)が起こっていると考えられる。同定できたマーカー候補ペプチドについては、安定同位体標識ペプチドを作成し、定量を行う。

Research Products

• [Journal Article] Proteome analysis of the rat pancreas by the chronic alcohol dosage. (2009)
  • Author(s): Mamoru Satoh, Eri Haruta-Satoh, Mako Yamada, Fumio Nomura
  • Journal Title: Alcohol And Biomedical Research 28 Pages: 16-20
• [Journal Article] Proteome analysis of a rat model of alcoholic liver injury. (2009)
  • Author(s): Mako Yamada, Mamoru Satoh, Kazuyuki Sogawa, Fumio Nomura
  • Journal Title: Alcohol And Biomedical Research 28 Pages: 21-25
• [Journal Article] Clinical application of alternative splicing form of c-myc suppressor FUSE-binding protein-interacting repressor for cancer detection and treatment. (2009)
  • Author(s): Matsushita K, Kajiwara T, Itoga S, Satoh M, Sogawa K, Umemura H, Sawai S, Nishimura M, Tamura M, Tanaka N, Shimada H, Tomonaga T, Nomura F.
  • Journal Title: 臨床病理 57(12) Pages: 1151-1158
• [Journal Article] A simple and highly reproducible method for discovering potential disease markers in low abundance serum proteins. (2009)
  • Author(s): YUsuke K, Tomoyuki F, Mamoru S, Hiroki T, Takashi M, Tadakazu M, Ybshio K.
  • Journal Title: Journal of Electrophoresis 53 Pages: 13-18
  • Peer Reviewed
• [Journal Article] YKL-40 identified by proteomic analysis as a biomarker of sepsis. (2009)
  • Author(s): Hattori N, Oda S, Sadahiro T, Nakamura M, Abe R, Shinozaki K, Nomura F, Tomonaga T, Matsushita K, Kodera Y, Sogawa K, Satoh M, Hirasawa H.
  • Journal Title: Shock 32(4) Pages: 393-400
  • Peer Reviewed
• [Journal Article] Nm23-Hl is responsible for SUMO-2-involved DNA synthesis induction after X-ray irradiation in human cells. (2009)
  • Author(s): Guo WZ, Sugaya S, Satoh M, Tomonaga T, Nomura F, Hiwasa T, Takiguchi M, Kita K, Suzuki N.
  • Journal Title: Archives of biochemistry and biophysics 486(1) Pages: 81-87
  • Peer Reviewed
• [Journal Article] A search for novel markers of alcohol abuse using magnetic beads and MALDI-TOF/TOF mass spectrometry. (2009)
  • Author(s): Sogawa K, Satoh M, Kodera Y, Tomonaga T, Iyo M, Nomura F.
  • Journal Title: Proteomics-Clinical Applications 3(7) Pages: 821-828
  • Peer Reviewed
• [Journal Article] Serum levels of complement C4 fragments correlate with disease activity in multiple sclerosis: proteo- mic analysis. (2009)
  • Author(s): Sawai S, Umemura H, Mori M, Satoh M, Hayakawa S, Kodera Y, Tomonaga T, Kuwabara S, Nomura F.
  • Journal Title: Journal of neuroimmunology 218 Pages: 112-115
  • Peer Reviewed
• [Journal Article] Effects of the time intervals between venipuncture and ser- um preparation for serum peptidome analysis by matrix-assisted laser desorption/ionizationtime-of -flight mass spectrometry. (2009)
  • Author(s): Umemura H, Nezu M, Kodera Y, Satoh M, Kimura A, Tomonaga T, Nomura F.
  • Journal Title: Clinica chimica acta 406 Pages: 179-180
  • Peer Reviewed
• [Journal Article] Involvement o f aldolase A in X-ray resistance of human HeLa and UV(r)-l cells (2008)
  • Author(s): Lu J, Suzuki T, Satoh M, Chen S, Tomon a ga T, Nomura F, Suzuki N.
  • Journal Title: Biochemical and biophysical research communications 369 Pages: 948-952
  • Peer Reviewed
• [Journal Article] Annexin II, a novel HSP27-interacted protein, is invol ved in resistance to UVC-induced cell deat h in human APr-l cells. (2008)
  • Author(s): Tong XB, Kita K, Karata K, Zhu CL, Sug aya S, Ichimura Y, Satoh M, Tomonaga T, Nomura F, Jin YH, Suzuki N.
  • Journal Title: Photochemistry an d Photobiology 84(6) Pages: 1455-1461
  • Peer Reviewed
• [Journal Article] Aberr ant promoter hypermethylation in serum D NA from patients with silicosis. (2008)
  • Author(s): Umemura S, Fujimoto N, Hiraki A, Gemba K, Takigawa N, Fujiwara K, Fujii M, Um emura H, Satoh M, Tabata M, Ueoka H, Kiura K, Kishimoto T, Tanimoto M.
  • Journal Title: Carcinogen esis 16 Pages: 1845-1849
  • Peer Reviewed
• [Presentation] 慢性的アルコール投与ラット膵臓のプロテオーム解析 (2009)
  • Author(s): 佐藤守
  • Organizer: アルコール医学生物学研究会
  • Place of Presentation: 三井ガーデンホテル千葉
  • Year and Date: 2009-11-13
• [Presentation] プロテオーム解析をもっと身近に (2009)
  • Author(s): 佐藤守
  • Organizer: 日本生化学会(シンポジウム)
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-24
• [Presentation] Plectin-l promotes migration and invasion of cancer cells and is a novel prognostic marker of head and neck cancer. (2009)
  • Author(s): Katada K, Tomonaga T, Matsushita K, Sato h M, Hanazawa T, Kodera Y, Okamoto Y Nomura F.
  • Organizer: 68th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-03
• [Presentation] Ide tification of new biomarker for early pancreatic cancer by three step serum proteome analysis. (2009)
  • Author(s): Abulaizi M, Tomonaga T, Sogawa K, Satoh M, Wubuli J, Matsushita K, Kodera Y, Nomura F.
  • Organizer: 68th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-10-03
• [Presentation] 慢性的アルコール摂取におけるラット膵臓のプロテオーム解析 (2008)
  • Author(s): 佐藤守
  • Organizer: アルコール医学生物学研究会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-09-15