2009 Fiscal Year Final Research Report
Elucidate the pathogenic mechanism of Helicobacter pyloriassociated chronic idiopathic thrombocytopenic purpura
| Project/Area Number |
20790415
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Kochi University |
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Principal Investigator |
NORIHITO Morimoto Kochi University, 医学部・附属病院, 臨床検査技師 (60398055)
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| Project Period (FY) |
2008 – 2009
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| Keywords | Helicobacter pylori (H. pylori) / 慢性特発性血小板減少性紫斑病(cITP) / 血小板低分子蛋白 / 免疫複合体 / 菌体膜蛋白(OMP) |
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| Research Abstract |
We reported the possibility that the immunocomplex consisting of bacterial 17-kDa protein, platelet and anti-H. pylori antibodies were involved in development of H. pylori-associated chronic idiopathic thrombocytopenic purpura (cITP). The H. pylori outer membrane protein (OMP) was identified among 11 candidates as one of bacterial factors involved in development of H. pylori - associated cITP. The recombinant fusion protein (rOMP) prepared was immunized with a rabbit to obtain polyclonal antibody. The specificity of the antibody was confirmed and this antibody could detect the OMP among individual H. pylori strains. The OMP was identified as one of the 17-kDa proteins of H. pylori, which binds to platelet, leading to aggregation.
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