2010 Fiscal Year Final Research Report
Development of simple mass screening method for the diagnosis of organic aciduria
| Project/Area Number |
20790417
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Fukuoka University |
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Principal Investigator |
YOSHIDA Hideyuki Fukuoka University, 薬学部, 准教授 (20301690)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 分析化学 / マススクリーニング / ジカルボン酸 / 有機酸代謝異常症 / エキシマー蛍光 / 蛍光誘導体化 / 生体計測 |
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| Research Abstract |
A simple screening method of organic aciduria by fluorometric measurement of total dicarboxylic acids inhuman urine is developed. This method is based on an intramolecular excimer-forming fluorescence derivatization with a pyrene reagent. This technique is so selective that it permits fluorometric measurement of total amount of dicarboxylic acids by the direct derivatization of diluted urine samples. The same reaction mixture was also served as a high-performance liquid chromatographic sample for the separative determination of individual dicarboxylic acids.
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