Development of diagnosis for asphyxia using 2-dimensional electrophoresis

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790461
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Legal medicine
• Research Institution: Nagasaki University
• Principal Investigator: IKEMATSU Kazuya Nagasaki University, 大学院・医歯薬学総合研究科, 准教授 (80332857)
• Project Period (FY): 2008 – 2009
• Keywords: 法医病理学

Research Abstract

We explored a specific protein which responded to hypoxia on an animal model of asphyxia with 2-Dimentioanl electrophoresis. Mice were anaesthetized after which hypoxia was induced by neck ligation for 30 min, then were killed by decapitation, and the brains were dissected either immediately (H-0) or 60 min (H-60) after death. Control mice without ligation of the neck were killed by decapitation under anaesthesia immediately (C-0) or 60 min (C-60) after death.
In forebrain, about 100 spots were observed. 2 spots were increased in H-0 and H-60. In addition, other 2 spots were increased. In cerebellum, about 100 spots were observed. 2 spots were increased in H-0 and H-60. In addition, other 2 spots were increased.
These increased spots might be asphyxia response proteins.