2009 Fiscal Year Final Research Report
Research on molecular mechanism of hepatic stem cell proliferation and development of mouse models for stem cell transplantation
| Project/Area Number |
20790485
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KAKINUMA Sei The University of Tokyo, 大学院・医歯学総合研究科, 寄附講座教員 (30372444)
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| Research Collaborator |
KAMIYA Akihide 東京大学, 医科学研究所, 助教 (30321904)
SAKAMOTO Naoya 東京医科歯科大学, 大学院・医歯学総合研究科, 寄附講座准教授 (10334418)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 再生医学 / 肝細胞 / 幹細胞 / 発生・分化 / 細胞移植 |
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| Research Abstract |
The first objective of this research project is clarification of molecular mechanisms in proliferation of primary mouse hepatic stem/progenitor cells (HSPCs). Primary mouse HSPCs were sorted using surface markers, and were infected with a viral vector expressing a target gene, then were cultured. Our data demonstrated that transcription factor Prox-1 enhances the proliferation of HSPCs, and Sall4 regulates the cell-fate determination of HSPCs. Second objective is development of mouse models for stem cell transplantation. The HSPCs were transplanted into liver-injured recipient mice. Our data showed that the recipient livers were reconstituted by donor HSPCs, and that donor cells were engrafted in long-term.
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[Journal Article] Lnk/Sh2b3 regulates integrinαIIbβ3 outside-in signaling in platelets leading to stabilization of developing thrombus in vivo.2010
Author(s)
Takizawa H, Nishimura S, Takayama N, Oda A, Nishikii H, Morita Y, Kakinuma S, Yamazaki S, Okamura S, Tamura N, Goto S, Sawaguchi A, Manabe I, Takatsu K, Nakauchi H, Takaki S, Eto K
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Journal Title
J Clin Invest 120
Pages: 179-190
Peer Reviewed
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[Journal Article] Comparison of HCV-associated gene expression and cell signaling pathways in cells with or without HCV replicon and in replicon-cured cells.2010
Author(s)
Nishimura-Sakurai Y, Sakamoto N,Mogushi K, Nagaie S, Nakagawa M,Itsui Y, Sekine-Osajima Y,Tasaka-Fujita M, Onuki-Karakama Y,Suda G, Mishima M, Yamamoto M,Ueyama M, Funaoka Y, Watanabe T,Chen CH, Kakinuma S, Tsuchiya K,Tanaka H, Enomoto N, Watanabe M
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Journal Title
J Gastroentorol (in press)
Peer Reviewed
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[Journal Article] Mutations in the interferon sensitivity determining region and virological response to combination therapy with pegylated-interferon alpha 2b plus ribavirin in patients with chronic hepatitis C-1b infection.2010
Author(s)
Nakagawa M, Sakamoto N, Ueyama M, Mogushi K, Nagaie S, Itsui Y, Azuma S, Kakinuma S, Tanaka H, Enomoto N, Watanabe M
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Journal Title
J Gastroentorol (in press)
Peer Reviewed
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[Journal Article] Antiviral effects of the interferon-induced protein GBP-1 and its interaction with the hepatitis C virus NS5B protein.2009
Author(s)
Itsui Y, Sakamoto N, Kakinuma S, Nakagawa M, Sekine-Osajima Y, Tasaka-Fujita M, Nishimura-Sakurai Y, Suda G, Karakama Y, Mishima K, Yamamoto M, Watanabe T, Ueyama M, Funaoka Y, Azuma S, Watanabe M
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Journal Title
Hepatology 50
Pages: 1727-37
Peer Reviewed
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