2009 Fiscal Year Final Research Report

Role of Gene33/RALT in oxidative stress-induced signal transduction in cardiac cells.

Research Project

Project/Area Number	20790557
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Circulatory organs internal medicine
Research Institution	Kanazawa Medical University
Principal Investigator	TAKEDA Kenji Kanazawa Medical University, 医学部, 助教 (90340009)
Project Period (FY)	2008 – 2009
Keywords	分子心臓病態学 / 蛋白質間相互作用 / 心筋細胞 / Mig-6
Research Abstract	We found that rat embryonic heart-derived H9c2 cells treated with oxidative stress exhibited a significant induction in Ralt mRNA and protein. Stable overexpression of Ralt in H9c2 cells prevented HB-EGF-induced signal transduction. To identify endogenous proteins that bind to Ralt in cardiac cells, we carried out a proteomic analysis and found that Ralt specifically bound to several different isoforms of 14-3-3 proteins independently of its phosphorylation status.

(3 results)