2009 Fiscal Year Final Research Report
Detection of tumor-derived mutations in circulating DNA
| Project/Area Number |
20790565
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Respiratory organ internal medicine
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
KIMURA Hideharu Kanazawa University, 医学部, 講師 (40444202)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Keywords | 癌 / 遺伝子変異 / 血中DNA |
|---|
| Research Abstract |
Patients with EGFR mutations were enrolled in this study. The concentration of the DNA extracted was higher in the patients' serum than in their plasma. The rate of EGFR mutation detection was higher, in their DNA from plasma, and by the Scorpion ARMS method. The detection rate was higher when whole genome amplification was performed.A larger study and the establishment of a more sensitive assay is needed to achieve the purpose of this study.
|
