The Metabolome Analysis: Association with pathogenesis of COPD in Leukocyte

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790575
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Keio University
• Principal Investigator: TAKAMIYA Rina Keio University, 医学系研究科, 特任研究員(常勤) (70365419)
• Project Period (FY): 2008 – 2009
• Keywords: COPD / 白血球 / 赤血球 / メタボローム解析 / 活性酸素 / ペントースリン酸回路

Research Abstract

申請者は、キャピラリー電気泳動-質量分析装置を用いたメタボローム解析より、タバコ煙抽出液は、細胞内糖代謝流速を解糖系からペントースリン酸回路にシフトさせ、ヌクレオチド生成も亢進させ、細胞内糖代謝応答に異常を引き起こす事で、白血球の機能や生存を制御している可能性が示唆する可能性を示した。そのため、この回路の異常が白血球の機能異常を齎し、慢性閉塞性肺疾患(COPD)の病態修飾に寄与すると考えられた。

Research Products

• [Journal Article] Levy The Anti-Inflammatory and Proresolving Mediator Resolvin E1 Protects Mice from Bacterial Pneumonia and Acute Lung Injury (2010)
  • Author(s): Hiroyuki Seki, Koichi Fukunaga, Arita, Makoto H, iroyuki Arai, Hiroki Nakanish, Ryo Taguchi, Toru Miyasho, Rina Takamiya, Koichiro Asano, Akitochi Ishizaka, Jyunzo Takeda, Bruce D
  • Journal Title: The Journal of Immunology 184 Pages: 836-843
  • Peer Reviewed
• [Journal Article] Rescue of anaemia and autoimmune responses in SOD1-deficient mice by transgenic expression of human SOD1 in erythrocytes (2009)
  • Author(s): Yoshito Iuchi, Futoshi Okada, Rina Takamiya, Noriko Kibe, Satoshi Tsunoda, Osamu Nakajima, Kazuyo Toyoda, Ritsuko Nagae, Makoto Suematsu, Tomoyoshi Soga, Koji Uchida, Jyunichi Fujii
  • Journal Title: Biochemical Journal 422 Pages: 313-320
  • Peer Reviewed
• [Journal Article] Perrella High-mobility group box 1 contributes to lethality of endotoxemia in heme oxygenase-1-deficient mice (2009)
  • Author(s): Rina Takamiya, Chi-Chih Hung, Sean R. Hall, Koichi Fukunaga, Takashi Nagaishi, Toshitaka Maeno, Caroline Owen, Alvaro A. Macias, Laura E. Fredenburgh, Akitoshi Ishizaka, Richard S. Blumberg, Rebecca M. Baron, Mark A.
  • Journal Title: American Journal of Respiratory Cell and Molecular Biology 41 Pages: 129-135
  • Peer Reviewed
• [Journal Article] High mobility group A1 protein mediates human nitric oxide synthase 2 gene expression (2008)
  • Author(s): Rina Takamiya, Rebecca M. Baron, Shaw-Fang Yet, Matthew D. Layne, Mark A. Perrella
  • Journal Title: FEBS Letters 582 Pages: 810-814
  • Peer Reviewed
• [Presentation] Cigarette smoke extract induces bisecting-GlcNAc modification in RAW264. 7 murine macrophage (2009)
  • Author(s): Rina Takamiya
  • Organizer: 20th Joint Glycobiology Meeting
  • Place of Presentation: ケルン、ドイツ
  • Year and Date: 20091109-20091110
• [Presentation] Cigarette smoke extract induces bisecting-GlcNAc modification in RAW264. 7 murine macrophage (2009)
  • Author(s): Rina Takamiya
  • Organizer: Japanese-Giessen GlycoWorkshop
  • Place of Presentation: ギーセン、ドイツ
  • Year and Date: 2009-11-07
• [Presentation] タバコ煙によるマクロファージ機能低下におけるレゾルビンE1の有用性 (2009)
  • Author(s): 高宮里奈
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] 網羅的代謝解析を中心としたアクロレインに曝露された赤血球の機能解明 (2008)
  • Author(s): 高宮里奈
  • Organizer: 第31回日本生化学会・分子生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Circulating levels of HMGB1 are exaggerated in HO-1 null mice during endotoxemia (2008)
  • Author(s): Rina Takamiya
  • Organizer: American Thoracic Society International Meeting 2008
  • Place of Presentation: トロント、カナダ
  • Year and Date: 2008-05-19