Comprehensive gene expression profiling analysis on diabetic nephropathy

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790592
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Nagoya University
• Principal Investigator: YOSHINARI Yasuda Nagoya University, 大学院・医学系研究科, 寄附講座准教授 (60432259)
• Project Period (FY): 2008 – 2009
• Keywords: 糖尿病性腎症 / DNAアレイ / バイオ・インフォマティクス

Research Abstract

A comprehensive gene expression analysis was performed by using mRNA extracted from human renal biopsy specimens after micro-disection, however signal sensitivity has still remained at the level of detection limit. In parallel, a bio-informatics analysis was conducted by using animal models of acute kidney injury. Comparison of the gene expression signatures of murine renal failure identified a shared transcriptome of 45 differentially regulated genes. 67% of these mRNAs were regulated in human renal disease and successfully segregated progressive DN from controls by clustering analysis.

Research Products

• [Journal Article] Modification of a new estimating equation for GFR, CKD-EPI equation for Japanese. (2010)
  • Author(s): Horio M, Imai E, Yasuda Y, Watanabe T, Matsuo S.
  • Journal Title: Am J Kid Dis. (in press)
• [Journal Article] Current status of estimated GFR (eGFR) equations for Asians and an approach to create a common eGFR equation. (2010)
  • Author(s): Matsuo S, Yasuda Y, Imai E, Horio M.
  • Journal Title: Nephrol. (in press)
• [Journal Article] Effects of oral cilostazol 100 mg BID on long-term patency after percutaneous transluminal angioplasty in patients with femoropopliteal disease undergoing hemodialysis: a retrospective chart review in Japanese patients. (2010)
  • Author(s): Ishii H, Kumada Y, Toriyama T, Aoyama T, Takahashi H, Tanaka M, Kamoi D, Kawamura Y, Yamada S, Hayashi M, Yasuda Y, Yuzawa Y, Maruyama S, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Clin Ther. 32(1) Pages: 24-33
• [Journal Article] Impact of renal function on coronary plaque composition. (2010)
  • Author(s): Miyagi M, Ishii H, Murakami R, Isobe S, Hayashi M, Amano T, Arai K, Yoshikawa D, Ohashi T, Uetani T, Yasuda Y, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Nephrol Dial Transplant. 25(1) Pages: 175-81
• [Journal Article] Prognostic values of C-reactive protein levels on clinical outcome after implantation of sirolimus-eluting stents in patients on hemodialysis. (2009)
  • Author(s): Ishii H, Toriyama T, Aoyama T, Takahashi H, Amano T, Hayashi M, Tanaka M, Kawamura Y, Yasuda Y, Yuzawa Y, Maruyama S, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Circ Cardiovasc Interv. 2(6) Pages: 513-8
• [Journal Article] Impact of microarousal associated with increased negative esophageal pressure in sleep-disordered breathing. (2009)
  • Author(s): Sukegawa M, Noda A, Yasuda Y, Nakata S, Sugiura T, Miyata S, Honda K, Hasegawa Y, Nakashima T, Koike Y.
  • Journal Title: Sleep Breath. (Epub ahead of print)
• [Journal Article] Collaborators developing the Japanese equation for estimated GFR. (2009)
  • Author(s): Matsuo S, Imai E, Horio M, Yasuda Y, Tomita K, Nitta K, Yamagata K, Tomino Y, Yokoyama H, Hishida A
  • Journal Title: Revised equations for estimated GFR from serum creatinine in Japan. Am J Kidney Dis. 53(6) Pages: 982-92
• [Journal Article] Aortic valvular calcification predicts restenosis after implantation of drug-eluting stents in patients on chronic haemodialysis (2009)
  • Author(s): Ishii H, Kumada Y, Toriyama T, Aoyama T, Takahashi H, Amano T, Yasuda Y, Yuzawa Y, Maruyama S, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Nephrol Dial Transplant. 24(5) Pages: 1562-7
• [Journal Article] on behalf of the collaborators for developing the Japanese equation for estimating GFR. (2009)
  • Author(s): Horio M, Imai E, Yasuda Y, Hishida A, Matsuo S
  • Journal Title: Simple sampling strategy for measuring inulin renal clearance. Clin Exp Nephrol. 13(1) Pages: 50-4
• [Journal Article] Aortic pressure augmentation as a marker of cardiovascular risk in obstructive sleep apnea syndrome. (2008)
  • Author(s): Noda A, Nakata S, Fukatsu H, Yasuda Y, Miyao E, Miyata S, Yasuma F, Murohara T, Yokota M, Koike Y.
  • Journal Title: Hypertens Res. 31(6) Pages: 1109-14
• [Journal Article] Cilostazol improves long-term patency after percutaneous transluminal angioplasty in hemodialysis patients with peripheral artery disease. (2008)
  • Author(s): Ishii H, Kumada Y, Toriyama T, Aoyama T, Takahashi H, Yamada S, Yasuda Y, Yuzawa Y, Maruyama S, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Clin J Am Soc Nephrol. 3(4) Pages: 1034-40
• [Journal Article] Sirolimus-eluting stents vs bare metal stents for coronary intervention in Japanese patients with renal failure on hemodialysis. (2008)
  • Author(s): Aoyama T, Ishii H, Toriyama T, Takahashi H, Kasuga H, Murakami R, Amano T, Uetani T, Yasuda Y, Yuzawa Y, Maruyama S, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Circ J. 72(1) Pages: 56-60