2009 Fiscal Year Final Research Report
Guided defferentiation of kidney precursor cells into specific cell lineages of the nephron
| Project/Area Number |
20790596
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Kidney internal medicine
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
KOBAYASHI Chiyoko Kumamoto University, 発生医学研究所, 助教 (20342785)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Keywords | 腎臓学 / 腎臓発生 / 前駆細胞 |
|---|
| Research Abstract |
The metanephros is the main filtrating organ of the mammalian organism. The basic structural and functional unit of the kidney is the nephron, which performs a multitude of tasks including blood filtration, re-absorption of salt, water and other compounds. These functions can be related to specific ‘units' in each nephron that are formed in a tightly controlled manner during development. Induction of the kidney occurs through reciprocal interactions between the ureteric bud and the metanephric mesenchyme. The mesenchymal cell sequentially form condensates, renal vesicles, comma- and S-shaped bodies, and terminal epithelia of glomeuli, proximal and distal tubules. To generate multiple cell lineages for kidney regeneration, there exist three obstacles to be overcome; (1) derivation of the renal progenitors; (2) expansion of the renal progenitors; and (3) control of lineage commitment of the renal progenitors toward differentiated cell types. The recent work on iPS formation suggests that a specific combination of multiple factors might be the most effective way to reprogram the cells. To develop of gene transducing methods into the kidney, we first tested lentivirus vector system.
|
-
[Journal Article] Kif26b, a kinesin family gene, regulates adhesion of the embryonic kidney mesenchyme2010
Author(s)
Yukako Uchiyama, Masaji Sakaguchi,Takeshi Terabayashi, Toshiaki Inenaga,Shuji Inoue, Chiyoko Kobayashi, NaokoOshima, Hiroshi Kiyonari, Naomi Nakagata,Yuya Sato, Kiyotoshi Sekiguchi, HiroakiMiki, Eiichi Araki, Sayoko Fujimura,Satomi Tanaka, Ryuichi Nishinakamura.
-
Journal Title
Proc. Natl. acad. Sci. USA. (in press)
Peer Reviewed
-
-
-
-
-
-
-
-
-
