2009 Fiscal Year Final Research Report
Clarification of urate transporter molecular complex and dynamic regulation in the kidney.
| Project/Area Number |
20790597
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kyorin University |
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Principal Investigator |
FUKUTOMI Toshiyuki Kyorin University, 医学部, 助教 (30439187)
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| Project Period (FY) |
2008 – 2009
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| Keywords | トランスポーター / 尿酸 / PDZタンパク質 / タンパク質間相互作用 / URAT1 / PDZK1 |
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| Research Abstract |
Renal urate transport system plays an important role in the regulation of plasma urate level. URAT1 is one of the major urate transporters in the kidney. Therefore, this study focused on elucidating URAT1-interacting protein(s) and their dynamic regulation through phosphorylation. URAT1 was shown to be bound to an organic anion transporter, OAT4, via a scaffold protein, PDZK1. In addition, increased transport activity was observed when each transporter interacted with PDZK1. Together with the fact that a phosphomimetic mutation in the URAT1 binding site of PDZK1 abolished the URAT1-PDZK1 interaction suggest a novel regulation mechanism for a URAT1 containing "urate transportsome".
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