LRRK2 associates with the membrane trafficking system.

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790624
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: Juntendo University
• Principal Investigator: HATANO Taku Juntendo University, 医学部, 助教 (60338390)
• Project Period (FY): 2008 – 2009
• Keywords: LRRK2 PARK8 / 家族性パーキンソン病 / 膜輸送 / Exocytosis

Research Abstract

Leucin-rich repeat kinase 2 (LRRK2) is causative protein of PARK8-linked familial Parkinson's disease and associates with the membrane trafficking system. In this study, we have explored whether LRRK2 influences the exocytosis in cultured cells. We revealed that knocking down LRRK2 expression disrupted the exocytosis of growth hormone in PC12 cells, which are rat adrenal pheocromocytoma cell line. However, pathogenic LRRK2 mutants could not influence the exocytosis, compared to wild LRRK2. Therefore, LRRK2 might participate in the membrane trafficking system, but we need further investigations to understand whether pathogenic LRRK2 mutants may affect the functions of exocytosis.

Research Products

• [Journal Article] Pathogenesis of familial Parkinson's disease: New insights based on monogenic forms of Parkinson's disease (2009)
  • Author(s): Hatano T, Kubo S-I, Sato S, Hattori N.
  • Journal Title: J Neurochem (in press)
  • Peer Reviewed
• [Journal Article] Fatigue in Japanese patients with Parkinson ' s disease: A study using Parkinson fatigue scale (2009)
  • Author(s): Okuma Y, Kamei S, Morita A, YoshiiF, Yamamoto T, Hashimoto S, Utsumi H, Hatano T, Hattori N, Matsumura M, Takahashi K, Nogawa S, Watanabe Y, Miyamoto T, Miyamoto M, Hirata K.
  • Journal Title: Mov Disord. (in press)
  • Peer Reviewed
• [Journal Article] Unmet needs of patients with Parkinson's disease: interview survey of patients and caregivers (2009)
  • Author(s): Hatano T, Kubo S-I, Shimo Y, Nishioka K, Hattori N.
  • Journal Title: J Int Med Res 37(3) Pages: 717-726
  • Peer Reviewed
• [Journal Article] Progress in the pathogenesis and genetics of Parkinson ' s disease (2008)
  • Author(s): Mizuno Y, Hattori N, Kubo S-I, Sato S, Nishioka K, Hatano T, Tomiyama H, Funayama M, Machida Y, Mochizuki H.
  • Journal Title: Philos Trans R Soc Lond B Biol Sci 363(1500) Pages: 2215-2227
  • Peer Reviewed
• [Journal Article] LRRK2 regulates synaptic vesicle endocytosis (2008)
  • Author(s): Shin N, Jeong H, Know J, Heo HY, Know JJ, Yun HJ, Kim CH, Tong Y, Shen J, Hatano T, Hattori N, Kim KS, Chang S, Seol W.
  • Journal Title: Exp Cell Res 314(10) Pages: 2055-2065
  • Peer Reviewed
• [Journal Article] The frequency of cardiac valvular regurgitation in Parkinson's disease (2008)
  • Author(s): Yamashiro K, Komine-Kobayashi M, Hatano T, Urabe T, Mochiuki H, Hattori N, Iwama Y, Daida H, Sakai M, Nakayama T, Mizuno Y.
  • Journal Title: Mov Disord 23(7) Pages: 935-941
  • Peer Reviewed
• [Presentation] パーキンソン病患者の服薬状況と治療費用について (2009)
  • Author(s): 波田野琢, 他
  • Organizer: 第50回日本神経学会総会
  • Place of Presentation: 仙台
  • Year and Date: 20090500
• [Presentation] Parkin knockout mouse脳を用いた蛋白質プロファイリング解析 (2009)
  • Author(s): 今井哲司, 波田野琢, 他
  • Organizer: 第50回日本神経学会総会
  • Place of Presentation: 仙台
  • Year and Date: 20090500
• [Presentation] Parkinの放出機構への影響の検討 (2009)
  • Author(s): 江口博人, 今泉美佳, 近岡洋子, 佐藤栄人, 波田野琢, 他
  • Organizer: 第50回日本神経学会総会
  • Place of Presentation: 仙台
  • Year and Date: 20090500
• [Presentation] DJ-1蛋白の細胞膜組織における局在に関する検討 (2008)
  • Author(s): 波田野琢, 他
  • Organizer: 第27回日本認知症学会
  • Place of Presentation: 前橋
  • Year and Date: 20081100
• [Presentation] パーキンソン病のunmet needs (2008)
  • Author(s): 波田野琢, 他
  • Organizer: 第49回日本神経学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20080500