Involvement of human lung mast cell activation through PAF receptor in severe asthma

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790700
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: KAJIWARA Naoki National Research Institute for Child Health and Development, 大学院・医学研究科, 博士研究員 (70453917)
• Co-Investigator(Renkei-kenkyūsha): OKAYAMA Yoshimichi 日本大学, 大学院・医学研究科, 准教授 (80292605)
• Project Period (FY): 2008 – 2009
• Keywords: マスト細胞 / 血小板活性化因子受容体 / ヒスタミン / 脱顆粒 / アレルギー

Research Abstract

In this study, we demonstrated for the first time that PAF induces histamine release from human lung mast cells but not human skin mast cells. PAF receptor on human peripheral blood-derived cultured mast cells coupled to pertussis toxin-sensitive Gi protein and activated PLC・2 and PLC・1 in response to PAF. These observations have important implications for our understanding of the mechanisms of acute allergic diseases, in particular asthma.

Research Products

• [Journal Article] Activation of human mast cells through the platelet-activating factor receptor (2010)
  • Author(s): Kajiwara N, Sasaki T, Bradding P, Cruse G, Sagara H, Ohmori K, Saito H, Ra C, Okayama Y.
  • Journal Title: J Allergy Clin Immunol 125(5) Pages: 1137-1145
  • Peer Reviewed
• [Presentation] Activation of platelet-activating factor receptor-coupled G・i leads to degranulation via phospholipase C・2 activation in human mast cells (2008)
  • Author(s): Okayama Y, Kajiwara N, Saito H, Ra C.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] PAF causes degranulation via PLC・2 activation in human mast cells (2008)
  • Author(s): Okayama Y, Kajiwara N, Ono Y, Saito H, Ra C.
  • Organizer: 第58回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-27