2009 Fiscal Year Final Research Report
Defective erythropoiesis and ribosomal protein genes : Analysis of zebrafish model for Diamond-Blackfan anemia
| Project/Area Number |
20790734
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | University of Miyazaki |
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Principal Investigator |
UECHI Tamayo University of Miyazaki, フロンティア科学実験総合センター, 研究員 (10381104)
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| Project Period (FY) |
2008 – 2009
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| Keywords | リボソームタンパク質遺伝子 / 造血異常 / ゼブラフィッシュ |
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| Research Abstract |
To investigate the pathogenic mechanism of Diamond-Blackfan anemia (DBA), I have developed a zebrafish DBA model by knocking down the ribosomal protein s19 gene using morpholino antisense oligo. Translational efficiency or production of total proteins in this model seemed almost same as control embryos. However, they showed the defective maturation of erythroid lineage by 2-3 days post fertilization. This suggests that there is an unknown mechanism that affect translation of specific factors, especially those involved in the erythroid pathway.
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