2010 Fiscal Year Final Research Report
Detection of imprinting abnormalities in rhabdomyosarcoma patients using tumor and serum DNA.
| Project/Area Number |
20790738
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
KIKUCHI Ken Kyoto Prefectural University of Medicine, 医学研究科, 助教 (40453104)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 横紋筋肉腫 / インプリンティング / IGF2 / 血清遊離DNA / H19 |
|---|
| Research Abstract |
In RMS cell lines and RMS tumor samples, we were able to detect a methylation abnormality in the imprinting center, in other words, imprinting abnormalities in IGF2 and H19 by Melting Curve Analysis. In RMS tumor samples, it was suggested that imprinting abnormalities correlate with the expression of IGF2 mRNA. Furthermore, we established a method to detect imprinting abnormalities in IGF2 and H19 in serum DNA.
|
