2010 Fiscal Year Final Research Report
Molecular analyses of ER stress in childhood nephrotic syndrome
| Project/Area Number |
20790740
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Wakayama Medical University |
|---|
Principal Investigator |
SHIMA Yuko Wakayama Medical University, 医学部, 助教 (60433364)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | ネフローゼ症候群 / 小胞体ストレス / シクロスポリン |
|---|
| Research Abstract |
To clarify the presence of ER stress and a role of it in children with nephrotic syndrome, we obtained renal biopsy specimens in children with nephrotic syndrome. We performed real-time PCR to investigate the presence of ER stress and apoptosis. We analyzed EIF2α, GRP78, GRP94, CHOP and BAD. The amount of each gene amplification was examined before and after the administration of cyclosporine. The patients with cyclosporine showed significant increase of amplification of GRP78, GRP94, BAD, and EIF2α, suggesting that the use of cyclosporine is a factor provoking ER stress. The amplification of BAD increased significantly after the administration of cyclosporine, suggesting that the use of cyclosporine may induce apoptosis through ER stress.
|
-
[Journal Article] Epithelial-to-mesenchymal transition in cyst lining epithelial cells in an orthologous PCK rat model of autosomal-recessive polycystic kidney disease.2011
Author(s)
Togawa H, Nakanishi K, Mukaiyama H, Hama T, Shima Y, Sako M, Miyajima M, Nozu K, Nishii K, Nagao S, Takahashi H, Iijima K, Yoshikawa N.
-
Journal Title
Am J Physiol Renal Physiol. 300(2)
Pages: F511-520
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
[Presentation] Snaill Is Involved in Epithelial-to-Mesenchymal Transition (EMT) in Cyst-Lining Epithelial.2009
Author(s)
Togawa H, Nakanishi K, Shima Y, Sako M, Miyajima M, Nozu K, Iijima K, Yoshihara D, Nagao S, Yoshikawa N
Organizer
42^<nd> Annual Meeting of the American Society of Nephrology
Place of Presentation
San Diego, USA
Year and Date
20091029-20091101
-
-
-
-
-
[Presentation] Disappearance of glomerular IgA deposits in severe childhood IgA nephropathy after 2-yr combination/predonisolone therapy.2008
Author(s)
Shima Y, Nakanishi K, Togawa H, Obana M, Nozu K, Tanaka R, Sasaki S, Iijima K, Yoshikawa N.
Organizer
J Am Soc Nephrol, 2008 41^<st> Annual Meeting of the American Society of Nephrology
Place of Presentation
Philadelphia, USA
Year and Date
20081104-20081109
-
-
