2009 Fiscal Year Final Research Report
The role of ALF4, a transcription factor, in the regulation of growth hormone secretion in the hypothalamus
| Project/Area Number |
20790742
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
KOMORI Tadasuke Wakayama Medical University, 医学部, 助教 (90433359)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 視床下部 / 転写因子 / グレリン / AMP-activated protein kinase / 摂食 |
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| Research Abstract |
AF5q31 expression was exclusively observed in growth hormone secretagogue-receptor (GHS-R)-expressing neurons during fasting. The intraperitoneal injection of ghrelin induced the expression of AF5q31 in the GHS-R-positive neurons of the arcuate nucleus and ventromedial hypothalamic nucleus. The decrease of AF5q31 expression in heterozygous mice attenuated the fasting-induced expression of AMPKα, as well as the expression of neuropeptide Y (NPY) and agouti-related peptide (AgRP) in the hypothalamus. Furthermore, the increase in fasting-induced food intake in heterozygous mice was less than that observed in wild-type mice. From these findings, ghrelin-induced AF5q31 in the hypothalamus appear to play an important role in the expression of AMPK, which may induce the expressions of NPY and AgRP, and thereby increase food intake.
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