2009 Fiscal Year Final Research Report
Dynamic analysis of the Nox family NADPH oxidases in the pathogenesis of Kawasaki disease
| Project/Area Number |
20790761
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | National Research Institute for Child Health and Development |
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Principal Investigator |
MORIYA Mie National Research Institute for Child Health and Development, 母児感染研究部, 共同研究員 (60470001)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 川崎病 / 循環器 / Nox / 酸化ストレス / TNF-α |
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| Research Abstract |
Potentiated generation of reactive oxygen spices (ROS) from neutrophil Nox2 by TNF-α was reported to contribute to ICAM-1 expression in vascular endothelial cells (VEC), which plays a key role in neutrophil extravasation and following aneurysm formation. However, this possibility was only concluded by indirect studies using reducing agents or inhibitors against Nox. In order to get a definite answer, we produced a mouse model of Kawasaki disease using LCWE, and then inspected it with Nox2-KO mice. The inspection studies clearly demonstrated that TNF-α induces ICAM-1 expression, directly affecting VEC without the aid of ROS from neutrophil Nox2.
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