Evaluation of Adrenal Function in Preterm Infants by Liquid Chromatography Tandem Mass Spectrometry based Serum Steroids Assay

2011 Fiscal Year Final Research Report

• Project/Area Number: 20790772
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Keio University
• Principal Investigator: MIWA Masayuki 慶應義塾大学, 医学部, 助教 (30383855)
• Project Period (FY): 2008 – 2011
• Keywords: 副腎機能 / 胎児副腎 / 早産児 / 超低出生体重児 / タンデム質量分析

Research Abstract

Fetal and adult adrenal glands markedly different to morphological and endocrinological. Therefore, accurate evaluation of adrenal function in neonate especially preterm infants is difficult. We measured serum steroids profile using liquid chromatography tandem mass spectrometry (LC/MSMS). That is the most reliable method to evaluate neonatal adrenal function. Result showed pattern of steroid secretory after birth was the same for term and preterm infants. Additionally 170Hpregnenolone and 170HP increased with younger gestational age, whereas cortisol was a small difference. To conclude, adrenal function is low in preterm infants.

Research Products

• [Journal Article] Late-onset Circulatory Collapse in Very Low-birthweight Infants : A Japanese Perspective (2009)
  • Author(s): Masayuki Miwa, Satoshi Kusuda, Kazushige Ikeda
  • Journal Title: NeoReviews 2009 Volume: 10 Pages: e381-e386
  • Peer Reviewed
• [Presentation] 副腎の発達正期産児と早産児 (2012)
  • Author(s): 三輪雅之
  • Organizer: 第2回名古屋新生児成長発達研究会
  • Place of Presentation: 愛知県・名古屋国際センタービル
  • Year and Date: 2012-01-28
• [Presentation] 早産児の血中アルドステロン濃度比較 (2011)
  • Author(s): 三輪雅之
  • Organizer: 第56回日本未熟児新生児学会
  • Place of Presentation: 東京都・東京国際フォーラム
  • Year and Date: 2011-11-14
• [Presentation] 新生児血中ステロイド一斉測定による週数別・AGA新生児副腎機能比較 (2011)
  • Author(s): 三輪雅之
  • Organizer: 第47回周産期・新生児学会
  • Place of Presentation: 北海道・札幌コンベンションセンター
  • Year and Date: 2011-07-12
• [Presentation] 正期産児および早産児における出生後血中ステロイドホルモン変化の比較 (2010)
  • Author(s): 三輪雅之
  • Organizer: 第46回周産期・新生児学会
  • Place of Presentation: 兵庫県・神戸国際会議場
  • Year and Date: 2010-07-13
• [Presentation] 30週以下の早産児における出生後早期の血中ステロイドホルモン変化 (2009)
  • Author(s): 三輪雅之
  • Organizer: 第45回周産期・新生児学会
  • Place of Presentation: 愛知県・名古屋国際会議場
  • Year and Date: 2009-07-14
• [Presentation] Serum Steroid Profile in Newborn : Comparison between Term and Preterm Infant by Using Liquid Chromatography/Mass Spectrometry/Mass Spectrometry (2008)
  • Author(s): Masayuki Miwa
  • Organizer: 49^<th> European Society for Paediatric Research
  • Place of Presentation: France, Nice
  • Year and Date: 2008-10-25
• [Book] 新生児内分泌ハンドブック (2008)
  • Author(s): 三輪雅之
  • Total Pages: 32-38
  • Publisher: メディカ出版