2009 Fiscal Year Final Research Report
Determinants of antimicrobial activity and salt-resistance of defensin
| Project/Area Number |
20790803
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Okayama University |
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Principal Investigator |
SHIRAFUJI Yoshinori Okayama University, 岡山大学病院, 助教 (90423285)
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| Project Period (FY) |
2008 – 2009
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| Keywords | デフェンシン / アルギニン / 抗菌活性 / 塩抵抗性 |
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| Research Abstract |
Human β-defensin (HBD) -3 has potent and salt-resistant antimicrobial activity against both gram-negativeand gram positive bacteria and this activity, while other HBDs possess antimicrobial activity against only gram-negative strains and it is attenuated under salt existence. We hypothesized that these characteristics are due to two arginine residues in the COOH-terminal of HBD-3 which is not involved in other HBD-3. To investigate, we designated two recombinant mutants of HBD-3; desR HBD-3 in which these two arginine residues were deleted from HBD-3 and NRR-HBD-3 in which these two arginine residues were shifted from COOH-terminal to NH2-terminal and esxamined their antimicrobial activities and salt-resistance. The results showed that these two argine residues are required to exist in the COOH-terminal in order to maintain the activity and salt-resistance of HBD-3.
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