2009 Fiscal Year Final Research Report
The role of Wnt signaling pathway in hair regeneration by using mouse adult hair follicle apithelial stem cells
| Project/Area Number |
20790812
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Nara Medical University |
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Principal Investigator |
OUJI Yukiteru Nara Medical University, 医学部, 助教 (50343421)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 皮膚炎症・再生学 / 毛包再生医学 |
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| Research Abstract |
Characterization and elucidation of the differentiation mechanism of stem cell in skin hair follicle (hair follicle epithelial stem cells : HFESCs) is important for understanding the differentiation of epithelial cells and hair follicle morphogenesis. Recently, it is thought that the Wnt signaling pathway is critical for regulating a number of basic cell functions, such as cell proliferation, differentiation, and migration, leading to morphogenesis and organogenesis, and we have already reported that the Wnt signal is important for hair reconstitution and hair growth in vitro and in vivo. In this study, we performed cell sorting of mouse HFESCs and examined the effects of the various Wnts (Wnt-3a, -5a, -10b, and -11) on HFESCs in vitro. Interestingly, only HFESCs cultured with Wnt-3a showed proliferated, and expressed stem cell markers compared with control condition. Furthermore, involvement of the canonical Wnt signal pathway was demonstrated by a TCF reporter (pTOPFLASH) assay. Our results suggest that Wnt-3a promotes the proliferation of HFESCs and may play an important role in HFESCs maintenance.
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