Comprehensive analysis of microbiota including unculturable microorganisms in various skin diseases

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790816
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Shimane University
• Principal Investigator: DEKIO Itaru Shimane University, 医学部, 講師 (80338128)
• Project Period (FY): 2008 – 2009
• Keywords: 皮膚感染症 / 微生物ゲノム / 皮膚科学

Research Abstract

In order to investigate comprehensive profiles of microbiota including unculturable microorganisms in various skin diseases, we examined sampling method from the skin lesions, culture conditions to grow microorganisms from the samples, and extraction method of microbial genomes from the samples. Swab-wash method was found to be suitable for sampling, and optimal culture conditions for culture analysis were discovered. However, we could not find extraction method for microbial genome of various skin microorganisms. As a secondary accomplishment, measurement of TARC level in stratum corneum in the tape-stripped skin samples is revealed to be useful to evaluate severity of skin lesion in atopic dermatitis patients.

Research Products

• [Journal Article] The weight of a finger-tip unit of ointment in 5-gram tubes. (2010)
  • Author(s): I. Dekio, E. Morita
  • Journal Title: Journal of Dermatological Treatment (in press)
  • Peer Reviewed
• [Journal Article] Stratum corneum TARC level is a new indicator of lesional skin inflammation in atopic dermatitis. (2010)
  • Author(s): E. Morita, H. Takahashi, H. Niihara, I. Dekio, Y. Sumikawa, Y. Murakami, H. Matsunaka.
  • Journal Title: Allergy (in press)
  • Peer Reviewed
• [Journal Article] N1 component reflects difference of terminal chords in three-chord sequences. (2009)
  • Author(s): S. Dekio-Hotta, T. Kojima, S. Karino, T. Yamasoba, I. Dekio, Y.M. Ito, H. Satake, K. Kaga
  • Journal Title: Neuroreport 20 Pages: 251-256
  • Peer Reviewed
• [Journal Article] Sparing of the bulge area could preserve intact lower portion of hair follicles in a case of tufted folliculitis. (2009)
  • Author(s): M. Miura, I. Dekio, Y. Yamasaki, M. Ohyama
  • Journal Title: J Eur Acad Dermatol Venereol 23 Pages: 87-89
  • Peer Reviewed
• [Journal Article] Sequence analysis of filaggrin gene by novel shotgun method in Japanese atopic dermatitis. (2008)
  • Author(s): T. Sasaki, J. Kudoh, T. Ebihara, A. Shiohama, S. Asakawa, A. Shimizu, A. Takayanagi, I. Dekio, C. Sadahira, M. Amagai, N. Shimizu
  • Journal Title: J Dermatol Sci 51 Pages: 113-120
  • Peer Reviewed
• [Presentation] Optimization of procedure for destructing mixture of bacteria and yeast for metagenomics. (2010)
  • Author(s): I. Dekio, H. Takahashi, Y. Sumikawa, E. Morita.
  • Organizer: Society for General Microbiology Spring 2010 Meeting
  • Place of Presentation: Edinburgh
  • Year and Date: 20100329-20100401
• [Presentation] The roles of the earwax type determination gene (ABCC11) in steroid resistance of atopic dermatitis patients. (2009)
  • Author(s): Y. Sumikawa, H. Takahashi, S. Kaneko, K. Kusatake, I. Dekio, M. Furumura, E. Morita
  • Organizer: The 34th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Fukuoka
  • Year and Date: 20091204-20091206
• [Presentation] アトピー性皮膚炎患者の表皮角層中プロテアーゼ. (2009)
  • Author(s): 横澤恵美子, 村上有美, 松中浩, 高橋仁, 新原寛之, 出来尾格, 森田栄伸
  • Organizer: 第108回日本皮膚科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 20090424-20090426
• [Presentation] Comprehensive characterization of skin microbiota in patients with atopic dermatitis and normal subjects using T-RFLP method. (2008)
  • Author(s): I. Dekio, M. Sakamoto, H. Hayashi, T. Ebihara, E. Morita, M. Suematsu, M. Amagai, Y. Benno
  • Organizer: The International Investigative Dermatology 2008
  • Place of Presentation: Kyoto
  • Year and Date: 20080500