Regulation of mast cell differentiation and function by Notch signaling and its involvement in allergic inflammatory disease

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790817
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Juntendo University
• Principal Investigator: NAKANO Nobuhiro Juntendo University, 大学院・医学研究科, 助教 (30420839)
• Project Period (FY): 2008 – 2009
• Keywords: アレルギー / マスト細胞 / Notch / 抗原提示 / Th2

Research Abstract

We demonstrated that mast cells constitutively expressed Notch1 and Notch2 on the cell surface, and Notch signaling induced the expression of MHC class II molecules and the costimulatory molecule OX40L on the mast cells. Furthermore, we showed that MHC class II+OX40L+ mast cells functioned as an antigen-presenting cell for CD4+ T cells and induced the differentiation of Th2. These results suggest that the mast cells as the antigen-presenting cells involve in the development of allergic diseases.

Research Products

• [Journal Article] Interaction of functional FCER2 promoter polymorphism and phenotype-associated haplotypes (2009)
  • Author(s): Potaczek DP, Wang QH, Sanak M, Tokura T, Matsuda H, Dziedzina S, Nakano N, Hara M, Ogawa H, Szczeklik A, Okumura K, Nishiyama C.
  • Journal Title: Tissue Antigens 74 Pages: 534-8
  • Peer Reviewed
• [Journal Article] FcεRIαgene -18483A>C polymorphism affects transcriptional activity through YY1 binding (2009)
  • Author(s): Potaczek DP, Maeda K, Wang QH, Nakano N, Kanada S, Stepien E, Branicka A, Fukai T, Hara M, Tokura T, Ogawa H, Undas A, Okumura K, Nishiyama C.
  • Journal Title: Immunogenetics 61 Pages: 649-55
  • Peer Reviewed
• [Journal Article] Involvement of PU. 1 in the transcriptional regulation of TNF-α (2009)
  • Author(s): Fukai T, Nishiyama C, Kanada S, Nakano N, Hara M, Tokura T, Ikeda S, Ogawa H, Okumura K.
  • Journal Title: Biochem Biophys Res Commun 388 Pages: 102-6
  • Peer Reviewed
• [Journal Article] Roles of PU. 1 in monocyte- and mast cell-specific gene regulation: PU. 1 transactivates CIITA pIV in cooperation with IFN-γ (2009)
  • Author(s): Ito T, Nishiyama C, Nakano N, Nishiyama M, Usui Y, Takeda K, Kanada S, Fukuyama K, Akiba H, Tokura T, Hara M, Tsuboi R, Ogawa H, Okumura K.
  • Journal Title: Int Immunol 21 Pages: 803-16
  • Peer Reviewed
• [Journal Article] Notch signaling confers antigen-presenting cell functions on mast cells (2009)
  • Author(s): Nakano N, Nishiyama C, Yagita H, Koyanagi A, Akiba H, Chiba S, Ogawa H, Okumura K.
  • Journal Title: J Allergy Clin Immunol 123 Pages: 74-81
  • Peer Reviewed
• [Journal Article] Opposite effects of PU. 1 on mast cell stimulation (2008)
  • Author(s): Niwa Y, Nishiyama C, Nakano N, Kamei A, Kato H, Kanada S, Ikeda S, Ogawa H, Okumura K.
  • Journal Title: Biochem Biophys Res Commun. 375 Pages: 95-100
• [Journal Article] Procyanidin C1 from apple extracts inhibits FcεRI-mediated mast cell activation (2008)
  • Author(s): Nakano N, Nishiyama C, Tokura T, Nagasako-Akazome Y, Ohtake Y, Okumura K, Ogawa H.
  • Journal Title: Int Arch Allergy Immunol 147 Pages: 213-21
  • Peer Reviewed
• [Presentation] Role of Notch receptors in acquisition of mast cell functions (2009)
  • Author(s): 中野信浩
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-02
• [Presentation] マスト細胞の形質獲得における各種Notchリガンドの役割 (2009)
  • Author(s): 中野信浩
  • Organizer: 第59回日本アレルギー学会秋季学術大会
  • Place of Presentation: 秋田
  • Year and Date: 2009-10-31
• [Presentation] 抗原提示細胞としてのマスト細胞の機能 (2009)
  • Author(s): 中野信浩
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] マスト細胞においてNotchシグナルにより誘導されるMHC class II発現のメカニズム (2008)
  • Author(s): 中野信浩
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] Notchシグナルによりマスト細胞が獲得する抗原提示細胞機能 (2008)
  • Author(s): 中野信浩
  • Organizer: 第58回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-27
• [Remarks]
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/atopy_center/index.html