2009 Fiscal Year Final Research Report
Functional analysis of GRIN family in the central nervous system
| Project/Area Number |
20790860
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Psychiatric science
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| Research Institution | Tsurumi University |
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Principal Investigator |
MOTOTANI Yasumasa Tsurumi University, 歯学部, 助教 (60421830)
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| Co-Investigator(Renkei-kenkyūsha) |
OKAMURA Tasashi 国立国際医療研究センター研究所, ヒト型動物開発研究質, 室長 (00333790)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 精神薬理学 |
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| Research Abstract |
We have studied about the roles of GRIN family, which binds to Gαi/o subunits, in the brain. In this study, we were able to generate both conditional GRIN1 KO mice and simple GRIN3 KO mice. Also, GRIN1 knockdown by lentivirus based RNAi system was successful. GRIN1 knockdown in retinoic acids induced neuronal P19 cells caused the reduction of GABAB2 receptor without the affect of Gαo expression. Furthermore, stimulation of cannabinoid receptor 1 (CB1) agonist could not activate the ERK pathway in these cells. On the other hand, ablation of GRIN3 in mice led to the decrease of both dopamine D1 and D2 receptors. These observations indicate that GRIN family plays the roles for GPCR function, such as stability and turn ov er in the cell membranes.
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