2009 Fiscal Year Final Research Report

BRM cocktail treatment using PSK and OK432 significantly up-regulates the migration activity in human dendritic cells without losing effective CTL induction

Research Project

Project/Area Number	20790934
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	General surgery
Research Institution	Fukushima Medical University
Principal Investigator	HOSHINO Mika Fukushima Medical University, 医学部, 助教 (00464511)
Project Period (FY)	2008 – 2009
Keywords	樹状細胞 / OK432 / PSK / 遊走能
Research Abstract	Dendritic cells (DCs) based cancer immunotherapies are widely applicable for many kinds of human cancers; however, patient outcomes are still not acceptable. One of the major factors for successful DCs immunotherapy is thought to be the maintenance of the migratory activity of matured DCs. In the present study we evaluated the effectiveness of protein bound polysaccharide PSK on OK432-activated DCs, in terms of maturation, migration and induction of cytotoxic T lymphocytes. OK432 treated DCs induced higher level of cytotoxic activity with poor migration ability. When DCs were treated with both OK432 and PSK, migration ability of the DCs were significantly high as compared to OK432 alone preserving the beneficial effect of OK432 treatment. Biological Response Modifier cocktail treatment with OK432 and PSK induced high level of migration activity in activated DCs, suggesting a potential protocol for more effective DC immunotherapy for cancer.

(4 results)