2009 Fiscal Year Final Research Report
Identification and characterization of hepatic cancer stem cells using oncogene-regulatory expressing transgenic mice
| Project/Area Number |
20790951
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
ISHII Takamichi Kyoto University, 再生医科学研究所, 研究員 (70456789)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 肝臓外科学 / 癌幹細胞 / 肝細胞癌 / 胆管細胞癌 |
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| Research Abstract |
that CSCs have self-renewal activities and multipotencies, which display a hierarchy in tumor tissues. This study aimed to demonstrate that alpha-fetoprotein (AFP)-producing cells in cholangiocarcinomas (CC) possessed CSC-like properties. The transgene vector that contained enhanced green fluorescent protein (EGFP) under the control of the human AFP enhancer/promoter was transfected into human CC cell lines. The AFP-EGFP-positive cells in RBE had self-renewal activity, differentiation ability, and tumorigenicity. The Notch signaling pathway was suggested to play an important role to maintain the stemness of the AFP-EGFP-positive cells. In addition, in order to explore the origin of CSCs, we successfully generated oncogene-regulatory expressing transgenic mice. One cell line was obtained from only fetal hepatocytes derived from the transgenic mice, thus suggesting that CSCs were generated from transformed stem cells.
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[Presentation] ALPHA-FETOPROTEIN PRODUCING CELLS AS A CANDIDATE FOR CANCER STEM CELLS OF CHOLANGIOCELLULAR CARCINOMA2009
Author(s)
Takamichi Ishii, Kentaro Yasuchika, Ken Fukumitsu, Sayuri Konishi, Masatoshi Kajiwara, Ryo Kamimura, Naoya Sasaki, Hirofumi Suemori, Norio Nakatsuji, Iwao Ikai, Shinji Uemoto
Organizer
4th Academic Surgical congress
Place of Presentation
Fort Meyers, USA
Year and Date
2009-02-05
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