2009 Fiscal Year Final Research Report
The role of Chemokine receptor CXCR3 in colon cancer metastasis
| Project/Area Number |
20790953
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
KAWADA Kenji Kyoto University, 医学研究科, 助教 (90322651)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 大腸癌 / ケモカイン受容体 / 転移 |
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| Research Abstract |
The SW480 cell line was derived from a primary Duke B colon cancer, whereas the SW620 cell line was derived from a lymph node metastasis of the same patient. Both CXCR3 and CXCR4 were much higher expressed in the SW620 than in the SW480. Cell migration, invasion and proliferation were induced by interaction of ligands with CXCR3 or CXCR4 in both cell lines. Importantly, CXCR4-induced migration and invasion were promoted when CXCR3 was pre-activated with its ligands in advance. These results suggest that CXCR3 can play a role in colon cancer metastasis by cooperating with CXCR4 synergistically. We established three stable cell lines using miRNA; CXCR3 knockout, CXCR4 knockout and CXCR3/CXCR4 double knockout cell lines. We have compared the metastatic potential of the three cell lines with the control cells by rectal transplantation model in nude mice.
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Research Products
(6 results)
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[Journal Article] Hepatic stellate cells promote liver metastasis of colon cancer cells by the action of SDF-1/CXCR4 axis.2009
Author(s)
Ryo Matsusue, Hajime Kubo, Shigeo Hisamori, Kae Okoshi, Hidekazu Takagi, Koya Hida, Keiko Nakano, Atsushi Itami, Kenji Kawada, Satoshi Nagayama, Yoshiharu Sakai
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Journal Title
Ann Surg Oncol Vol16
Pages: 2645-2653
Peer Reviewed
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