2009 Fiscal Year Final Research Report
Identification of a novel tumor-associated antigen, SPARC, as a possible target for diagnosis and therapy of scirrhous gastric cancers
| Project/Area Number |
20790962
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
IKUTA Yoshiaki Kumamoto University, 医学部・附属病院, 非常勤診療医師 (70452894)
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| Project Period (FY) |
2008 – 2009
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| Keywords | cDNAマイクロアレイ / SPARC / 分子標的療法 / 免疫療法 |
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| Research Abstract |
We identified HLA-A24-restricted and SPARC-derived CTL epitope peptides. By using these peptides, we tried to induce HLA-A24 (A*2402) -restricted and SPARC-reactive human CTLs, and demonstrate an antitumor immune response.The SPARC peptides-reactive CTLs were successfully induced from PBMCs of positive healthy donors and cancer patients by in vitro stimulation, and these CTLs exhibited cytotoxicity specific to cancer cells expressing both SPARC and HLA-A24. The adoptive transfer of the SPARC-specific CTLs could inhibit the tumor growth in NOD/SCID mice bearing human cancer cells expressing both HLA-A24 and SPARC.These findings suggest that SPARC is a potentially useful target candidate for cancer immunotherapy.
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