2009 Fiscal Year Final Research Report

Fluorescence diagnosis of lung cancer and malignant mesothelioma using orthotopic ijplamtation SCID mice model

Research Project

Project/Area Number	20790991
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Thoracic surgery
Research Institution	The University of Tokushima
Principal Investigator	TAKIZAWA Hiromitsu The University of Tokushima, 医学部・歯学部附属病院, 講師 (90332816)
Project Period (FY)	2008 – 2009
Keywords	蛍光診断 / 5-アミノレブリン酸 / タラポルフィン / SCIDマウス / 肺癌 / 悪性胸膜中皮腫 / リンパ節転移
Research Abstract	Human lung cancer cell line A549, Ma44-3 and human malignant mesothelioma cell line MST0211H were injected into left lung or left pleural cavity of severe combined immunodeficiency disease mice. The mice were administered 5-aminolevulinic acid or mono-L-aspartyl chlorine e6 1 to 3 weeks after the implantation. The mice were sacrificed several hours after the administration of these drugs. Their chest walls were opened and illuminated with blue light (405nm). Clear red fluorescence was observed in lung tumors, metastatic lymph nodes, and pleural tumors, even though the tumors were invisible macroscopically under white light. This result suggests possibility of clinical application of fluorescence diagnosis with intrapleural malignant tumors.