Pathophysiology of lymphatic stomata in the pathogenesis of cancerous pleural effusion and mechanisms of anchorage-independent growth of cancer

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790993
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Yokohama City University
• Principal Investigator: OSHIRO Hisashi Yokohama City University, 附属病院, 助教 (60381513)
• Research Collaborator: 大谷 修 富山大学, 大学院・医学薬学研究部, 教授 (90127548) ; 三浦 真弘 大分大学, 医学部, 講師 (50199957) ; 青葉 孝昭 日本歯科大学, 歯学部, 教授 (30028807) ; 島津 徳人 日本歯科大学, 歯学部, 講師 (10297947) ; 工藤 朝雄 日本歯科大学, 歯学部 ; 海老原 善郎 東京医科大学, 医学部, 名誉教授 ; 工藤 玄恵 東京医科大学, 医学部, 教授 (10130361) ; 芹澤 博美 東京医科大学, 医学部, 准教授 ; 吉濱 勲 東京医科大学, 医学部, 講師 ; 千島 隆司 横浜市立大学, 医学部, 准教授 (70438141) ; 前川 二郎 横浜市立大学, 医学部, 准教授 ; 長濱 清隆 横浜市立大学, 医学部, 助教 (00336538) ; 奥寺 康司 横浜市立大学, 医学部, 助教 (10326027) ; 深澤 由里 東邦大学, 医学部, 助教 (90392331) ; SUAMI Hiroo The University of Texas・MD Anderson Cancer Center, Assistant Professor
• Project Period (FY): 2008 – 2009
• Keywords: 肺靱帯 / リンパ管小孔 / 癌性胸水 / 胸膜播種 / リンパ行性転移 / 胸管 / 静脈角リンパ節 / Vascular endothelial growth factor (VEGF)

Research Abstract

Autopsy cases with carcinomas were analyzed in order to elucidate mechanisms of cancerous pleural effusion in humans. Chi-square test demonstrated that the frequencies of dissemination/invasion onto the pleura, pulmonary hilar lymph node metastasis, metastasis to the lymphatic stomata on the pulmonary ligament, and venous angle lymph node metastasis in cases with cancerous pleural effusion were all significantly higher than those in cases without cancerous pleural effusion; however, multiple logistic regression analysis demonstrated that among these variables, only the metastasis to the lymphatic stomata on the pulmonary ligament was a significantly independent predictive factor for cancerous pleural effusion. Among untreated cases with cancerous pleural effusion, Ki-67 and apoptosis indexes of cancer cells were not significantly different between primary lesions and metastatic lesions to the lymphatic stomata on the pulmonary ligament; however, VEGF-C immunohistochemistry demonstrated a potentially increased expression levels at the metastatic lesions to the lymphatic stomata compared with the primary lesions, and E-cadherin immunohistochemistry demonstrated a potentially decreased expression levels at the metastatic lesions to the lymphatic stomata compared with the primary lesions. These results raise a possibility that cancer metastasis to the lymphatic stomata plays a role in the pathogenesis of cancerous pleural effusion.

Research Products

• [Journal Article] Multiple lung nodules with kidney dysfunction. (2010)
  • Author(s): Hitomi Tanaka, Takuma Fujii, Takashi Kusaura, Akiko Kobayashi, Hisashi Oshiro, Takeshi Sasaki, Yutaka Yamaguchi, Kiyotaka Nagahama
  • Journal Title: Am J Kid Dis. (in press)
  • Peer Reviewed
• [Journal Article] Gallbladder Metastasis from Renal Cell Carcinoma. (2010)
  • Author(s): Kawahara T, Ohshiro H, SekiguchiZ, Furuya M, Namura K, Itoh H, Sano F, Kawaji K, Hayashi N, Makiyama K, Nakaigawa N, Ogawa T, Uemura H, Yao M, Kubota Y
  • Journal Title: Case Rep Oncol. 3 Pages: 30-34
  • Peer Reviewed
• [Journal Article] Neuroendocrine Carcinoma of the Bladder. (2010)
  • Author(s): Kawahara T, Yamanaka S, Ohshiro H, Sekiguchi Z, Namura K, Itou H, Sano F, Kita K, Hayashi N, Makiyama K, Nakaigawa N, Ogawa T, Uemura H, Yao M, Kubota Y
  • Journal Title: Case Rep Oncol. 3 Pages: 54-58
  • Peer Reviewed
• [Journal Article] A novel method for isolating podocytes using magnetic activated cell sorting. (2010)
  • Author(s): Murakami A, Oshiro H, Kanzaki S, Yamaguchi A, Yamanaka S, Furuya M, Miura S, Kanno H, Nagashima Y, Aoki I, Nagahama K
  • Journal Title: Nephrol Dial Transplant (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Urinary cytologic features of primary large cell neuroendocrine carcinoma of the urinary bladder. (2010)
  • Author(s): Oshiro H, Gomi K, Nagahama K, Nagashima Y, Kanazawa M, Kato J, Hatano T, Inayama Y
  • Journal Title: Acta Cytol. 54(3) Pages: 303-10
  • Peer Reviewed
• [Journal Article] Early Growth Response-1 Induces and Enhances Vascular Endothelial Growth Factor-A Expression in Lung Cancer Cells. (2010)
  • Author(s): Shimoyamada H, Yazawa T, Sato H, Okudela K, Ishii J, Sakaeda M, Kashiwagi K, Suzuki T, Mitsui H, Woo T, Tajiri M, Ohmori T, Ogura T, Masuda M, Oshiro H, Kitamura H
  • Journal Title: Am J Pathol. (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Metastatic tumor in the colon from renal cell carcinoma. (2010)
  • Author(s): Nozaki Y, Inamori M, Fujita K, Yoneda M, Kato S, Uchiyama T, Suzuki K, Watanabe S, Mawatari H, Iida H, Hosono K, Endo H, Sakamoto Y, Yoneda K, Takahashi H, Koide T, Tokoro C, Kobayashi N, Kirikoshi H, Shimamura T, Abe Y, Kubota K, Saito S, Oshiro H, Inayama Y, Nakajima A.
  • Journal Title: Intern Med. 49(7) Pages: 709
  • Peer Reviewed
• [Journal Article] Well-differentiated extraskeletal osteosarcoma arising from the retroperitoneum that recurred as anaplastic spindle cell sarcoma. (2010)
  • Author(s): Arai H, Rino Y, Nishii T, Yukawa N, Wada N, Oshiro H, Ishida T, Nakaigawa N, Masuda M
  • Journal Title: Case Report Med. Pages: 327591
  • Peer Reviewed
• [Journal Article] Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis. (2010)
  • Author(s): Machida H, Ito S, Hirose T, Takeshita F, Oshiro H, Nakamura T, Mori M, Inayama Y, Yan K, Kobayashi N, Yokota S
  • Journal Title: Nephrol Dial Transplant (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Imprint cytology of primary ethmoidal lipid-rich carcinoma combined with adipophilin immunocytochemistry. (2010)
  • Author(s): Oshiro H, Nagashima Y, Kanazawa M, Sagawa H, Yamanaka S, Inayama Y
  • Journal Title: Cytopathology (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Langerhans Cell Histiocytosis of the Stomach Mimicking Early Gastric Cancer. (2010)
  • Author(s): Nozaki Y, Oshiro H, Nakajima A
  • Journal Title: Clin Gastroenterol Hepatol (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Grade I lymphomatoid granulomatosis with increased uptake of [18F] fluorodeoxyglucose in positron emission tomography: a case report. (2009)
  • Author(s): Arai H, Oshiro H, Yamanaka S, Yukawa N, Wada N, Rino Y, Watanuki Y, Yamanaka S, Inayama Y, Lee J, Nakayama H, Masuda M
  • Journal Title: J Clin Exp Hematop 49(1) Pages: 39-44
  • Peer Reviewed
• [Journal Article] Ethmoidal lipid-rich carcinoma with focal glandular structures. (2009)
  • Author(s): Oshiro H, Nagao J, Nagashima Y, Taguchi T, Yamanaka S, Inayama Y
  • Journal Title: Arch Otolaryngol Head Neck Surg. 135(5) Pages: 511-4
  • Peer Reviewed
• [Journal Article] Case of sarcomatoid renal cell carcinoma developed in the chalked kidney (putty kidney). (2009)
  • Author(s): Komeya M, Sano F, Kagota M, Murakami T, Makiyama K, Miyoshi Y, Nakaigawa N, Ogawa T, Uemura H, Yao M, Ooshiro H, Nagashima Y, Kubota Y
  • Journal Title: Hinyokika Kiyo 55(5) Pages: 253-7
  • Peer Reviewed
• [Journal Article] Epithelioid angiomyolipoma of the kidney. (2009)
  • Author(s): Kato I, Inayama Y, Yamanaka S, Ohshiro H, Gomi K, Shirai S, Aoki I, Uemura H, Miyoshi Y, Kubota Y, Yao M, Nagashima Y
  • Journal Title: Pathol Int. 59(1) Pages: 38-43
  • Peer Reviewed
• [Journal Article] 化学療法が奏効した多発転移を有する2型S状結腸癌を合併した4型上行結腸癌の1例 (2009)
  • Author(s): 湯川寛夫, 利野靖, 菅野伸洋, 村上仁志, 松浦仁, 高田賢, 大城久, 影山裕, 益田宗孝
  • Journal Title: 日本外科系連合学会誌 34 Pages: 871-9
  • Peer Reviewed
• [Journal Article] 横隔膜上リンパ節転移で再発し胸腔鏡下に切除した胆管細胞癌の1例 (2009)
  • Author(s): 湯川寛夫, 利野靖, 荒井宏雅, 山中澄隆, 大城久, 益田宗孝
  • Journal Title: 日本内視鏡外科学会雑誌 14(2) Pages: 211-5
  • Peer Reviewed
• [Journal Article] Mucin-producing gallbladder adenocarcinoma with focal small cell and large cell neuroendocrine differentiation associated with pancreaticobiliary maljunction. (2008)
  • Author(s): Oshiro H, Matsuo K, Mawatari H, Inayama Y, Yamanaka S, Nagahama K, Endo I, Shimada H, Nakajima A, Kubota K
  • Journal Title: Pathol Int. 58(12) Pages: 780-6
  • Peer Reviewed
• [Journal Article] Differentiating sclerosing cholangitis caused by autoimmune pancreatitis and primary sclerosing cholangitis according to endoscopic duodenal papillary features. (2008)
  • Author(s): Kubota K, Kato S, Akiyama T, Yoneda M, Fujita K, Ogawa M, Inamori M, Kobayashi N, Saito S, Kakuta Y, Ohshiro H, Nakajima A
  • Journal Title: Gastrointest Endosc. 68(6) Pages: 1204-8
  • Peer Reviewed
• [Journal Article] Successful treatment of adrenocortical carcinoma with pulmonary metastasis in a child: report of a case. (2008)
  • Author(s): Arai H, Rino Y, Yamanaka S, Yukawa N, Wada N, Kato H, Yanagimachi M, Goto H, Oshiro H, Yamanaka S, Inayama Y, Lee J, Masuda M
  • Journal Title: Surg Today 38(10) Pages: 965-9
  • Peer Reviewed
• [Journal Article] Lung cancer associated with Sweet's syndrome: report of a case. (2008)
  • Author(s): Arai H, Rino Y, Yamanaka S, Suganuma N, Yukawa N, Wada N, Hara S, Hirokado M, Oshiro H, Masuda M
  • Journal Title: Surg Today 38(7) Pages: 639-43
  • Peer Reviewed
• [Journal Article] Endometrial adenocarcinoma without myometrial invasion metastasizing to the pancreas and masquerading as primary pancreatic neoplasm. (2008)
  • Author(s): Oshiro H, Miyagi Y, Kawaguchi Y, Rino Y, Arai H, Asai-Sato M, Nakayama H, Yamanaka S, Inayama Y, Fukushima N
  • Journal Title: Pathol Int. 58(7) Pages: 456-61
  • Peer Reviewed
• [Journal Article] Collagenous colitis in a Japanese woman: successful treatment with mesalazine. (2008)
  • Author(s): Suzuki K, Kawabe T, Takahashi H, Nakao S, Suzuki A, Inamori M, Iida H, Endo H, Akiyama T, Fujita K, Yoneda M, Abe Y, Kobayashi N, Kubota K, Oshiro H, Nakajima A
  • Journal Title: Digestion 77(3-4) Pages: 155-6
  • Peer Reviewed
• [Journal Article] 診断に難渋した甲状腺無色素性悪性黒色腫の1例 (2008)
  • Author(s): 西尾由紀子, 佐川弘美, 本野紀夫, 三田和博, 石井みどり, 北村和久, 大城久, 稲山嘉明
  • Journal Title: 日本臨床細胞学会神奈川県支部会誌 13(1) Pages: 42-6
  • Peer Reviewed
• [Presentation] 肝門部原発未分化癌の細胞学的所見 (2009)
  • Author(s): 西尾由紀子, 大城久, 山中正二, 北村和久, 三田和博, 本野紀夫, 佐川弘美, 金澤美千代, 中山崇, 稲山嘉明
  • Organizer: 第48回日本臨床細胞学会秋期大会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-10-31
• [Presentation] 子宮頸部細胞診が有用であった卵管癌と子宮頸部腺癌の重複癌の1例 (2009)
  • Author(s): 杉浦賢, 宮城悦子, 井畑穣, 平原史樹, 小林友紀, 稲山嘉明, 北村和久, 大城久, 山中正二, 古屋充子
  • Organizer: 第48回日本臨床細胞学会秋期大会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-10-31
• [Presentation] 副鼻腔原発lipid-rich carcinomaの細胞学的所見 (2009)
  • Author(s): 大城久, 長嶋洋治, 金澤美千代, 佐川弘美, 西尾由紀子, 長濱清隆, 小野響子, 中山崇, 山中正二, 稲山嘉明
  • Organizer: 第48回日本臨床細胞学会秋期大会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-10-30
• [Presentation] 膵管内乳頭状粘液性腫瘍(IPMT)12例の膵液細胞診の検討 (2008)
  • Author(s): 佐川弘美, 北村和久, 石井みどり, 三田和博, 本野紀夫, 西尾由紀子, 金澤美千代, 大城久, 山中正二, 稲山嘉明
  • Organizer: 第49回日本臨床細胞学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-06
• [Presentation] 腎上皮性腫瘍の新組織分類病理学と分子生物学的側面から (2008)
  • Author(s): 長嶋洋治, 大城久, 山中正二, 稲山嘉明
  • Organizer: 第49回日本臨床細胞学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-06
• [Presentation] 乳癌におけるaPKCλ/ι状態は病理型と関連する(The status of the aPKCλ/ιin breast cancer correlates with the pathological types)(英語) (2008)
  • Author(s): 長嶋洋治, 稲山嘉明, 佐々木毅, 大城久, 村上あゆみ, 山中正二, 河内香江, 野澤昭典, 青木一郎
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢市
  • Year and Date: 2008-05-15
• [Presentation] バーチャルスライドシステム横浜市大病院での運用状況 (2008)
  • Author(s): 稲山嘉明, 山中正二, 大城久, 新井信隆
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢市
  • Year and Date: 2008-05-15