Identification of BMP responsive osteoprogenitor cells in muscle tissue

2009 Fiscal Year Final Research Report

• Project/Area Number: 20791050
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Saitama Medical University
• Principal Investigator: FUKUDA Toru Saitama Medical University, 医学部, 助教 (20301492)
• Project Period (FY): 2008 – 2009
• Keywords: BMP / 骨形成 / トランスジェニック

Research Abstract

BMPs were identified as factors responsible for induction of bone formation. However, the BMP responsive osteoprogenitor cells were remains unknown. To identify these progenitors, we attempted to generate a ALK2(R206H) transgenic mice.

Research Products

• [Journal Article] Constitutively activated ALK2 and increased SMAD1/5 cooperatively induce bone morphogenetic protein signaling in fibrodysplasia ossificans progressiva (2009)
  • Author(s): Fukuda T, Kohda M, Kanomata K, Nojima J, Nakamura A, Kamizono J, Noguchi Y, Iwakiri K, Kondo T, Kurose J, Endo K, Awakura T, Fukushi J, Nakashima Y, Chiyonobu T, Kawara A, Nishida Y, Wada I, Akita M, Komori T, Nakayama K, Nanba A, Maruki Y, Yoda T, Tomoda H, Yu PB, Shore EM, Kaplan FS, Miyazono K, Matsuoka M, Ikebuchi K, Ohtake A, Oda H, Jimi E, Owan I, Okazaki Y, Katagiri T.
  • Journal Title: J. Biol. Chem. 284 Pages: 7149-7156
  • Peer Reviewed
• [Journal Article] Protein Phosphatase Magnesium-Dependent 1A-Mediated Inhibition of BMP Signaling is Independent of Smad Dephosphorylation (2009)
  • Author(s): Kokabu S, Nojima J, Kanomata K, Ohte S, Yoda T, Fukuda T, Katagiri T
  • Journal Title: J. Bone Miner. Res. (in press)
  • Peer Reviewed
• [Journal Article] miR-210 promotes osteoblastic differentiation through inhibition of AcvR1b (2009)
  • Author(s): Mizuno Y, Tokuzawa Y, Ninomiya Y, Yagi K, Yatsuka-Kanesaki Y, Suda T, Fukuda T, Katagiri T, Kondoh Y, Amemiya T, Tashiro H, Okazaki Y
  • Journal Title: FEBS Lett. 583 Pages: 2263-226
  • Peer Reviewed
• [Journal Article] Distinct function of 2 chromatin remodeling complexes that share a common subunit, Williams syndrome transcription factor (WSTF) (2009)
  • Author(s): Yoshimura K, Kitagawa H, Fujiki R, Tanabe M, Takezawa S, Takada I, Yamaoka I, Yonezawa M, Kondo T, Furutani Y, Yagi H, Yoshinaga S, Masuda T, Fukuda T, Yamamoto Y, Ebihara K, Li DY, Matsuoka R, Takeuchi JK, Matsumoto T, Kato S
  • Journal Title: Proc. Natl. Acad. Sci. U S A. 106 Pages: 9280-9285
  • Peer Reviewed
• [Journal Article] DRAGON, a GPI-anchored membrane protein, inhibits BMP signaling in C2C12 myoblasts (2009)
  • Author(s): Kanomata K, Kokabu S, Nojima J, Fukuda T, Katagiri T
  • Journal Title: Genes Cells 14 Pages: 695-702
  • Peer Reviewed
• [Journal Article] A unique mutation of ALK2, G356D, found in a patient with fibrodysplasia ossificans progressiva is a moderately activated BMP type I receptor (2008)
  • Author(s): Fukuda T, Kanomata K, Nojima J, Kokabu S, Akita M, Ikebuchi K, Jimi E, Komori T, Maruki Y, Matsuoka M, Miyazono K, Nakayama K, Nanba A, Tomoda H, Okazaki Y, Ohtake A, Oda H, Owan I, Yoda T, Haga N, Furuya H, Katagiri T
  • Journal Title: Biochem. Biophys. Res. Commun. 377 Pages: 905-909
  • Peer Reviewed
• [Journal Article] miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation (2008)
  • Author(s): Mizuno Y, Yagi K, Tokuzawa Y, Kanesaki-Yatsuka Y, Suda T, Katagiri T, Fukuda T, Maruyama M, Okuda A, Amemiya T, Kondoh Y, Tashiro H, Okazaki Y
  • Journal Title: Biochem. Biophys. Res. Commun. 368 Pages: 267-272
  • Peer Reviewed
• [Presentation] WntとBMPリガンドによる相乗的な骨芽細胞の誘導機構 (2009)
  • Author(s): 福田亨
  • Organizer: 第32回BMP日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-12
• [Presentation] 筋組織内に異所性骨化を生じる遺伝性難病Fibrodysplasia Ossificans Progressiva (FOP) (2009)
  • Author(s): 福田亨
  • Organizer: 第10回運動器科学研究会
  • Place of Presentation: 東京ステーションコンファレンス(東京都)
  • Year and Date: 2009-09-18
• [Presentation] Functional Analysis of Mutant ALK2 Receptors Found in Fibrodysplasia ossificans progressiva (FOP) (2009)
  • Author(s): 福田亨
  • Organizer: 31st ASBMR (American Society for Bone and Mineral Research) annual meeting
  • Place of Presentation: コロラドコンベンションセンター(アメリカ
  • Year and Date: 2009-09-12
• [Presentation] Fibrodysplasia ossificans progressiva (FOP)で新たに同定されたALK2変異体の機能解析 (2009)
  • Author(s): 福田亨
  • Organizer: 第16回BMP研究会
  • Place of Presentation: 大阪大学中之島センター(大阪府)
  • Year and Date: 2009-07-26
• [Presentation] Fibrodysplasia ossificans progressiva (FOP)で同定されたALK2変異体の解析 (2009)
  • Author(s): 福田亨
  • Organizer: 第27回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2009-07-25