2009 Fiscal Year Final Research Report
Cell therapy for spinal cord injury using induced pluripotent stem cells.
| Project/Area Number |
20791052
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Keio University |
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Principal Investigator |
TSUJI Osahiko Keio University, 医学部, 助教 (70424166)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 脊椎損傷 / iPS細胞 / 神経幹細胞 |
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| Research Abstract |
Here we demonstrated the directed neural differentiation of murine iPS cells and examined their therapeutic potential in a mouse spinal cord injury (SCI) model. 'Safe' iPS-derived neurospheres, which had been pre-evaluated as non-tumorigenic by their transplantation into NOD/SCID mouse brain, produced neurons, astrocytes, and oligodendrocytes in vitro. Furthermore, when the 'safe' iPS-derived neurospheres were transplanted into the spinal cord 9 days after contusive injury, they differentiated into all three neural lineages without forming teratomas or other tumors. They also participated in re-myelination and induced the axonal re-growth of host 5HT+ serotonergic fibers, promoting locomotor function recovery. These results suggest that pre-evaluated safe' PS clone-derived neural stem/progenitor cells may be a promising cell source for transplantation therapy for SCI.
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[Presentation] Cell therapy for spinal cord injury by neural stem/progenitor cells derived from induced pluripotent stem cells2008
Author(s)
Osahiko Tsuji, Kyoko Miura, Masaya Nakamura, Yohei Okada, Narihito Nagoshi, Kazuya Kitamura, Gentaro Kumagai, Masahiko Mukaino, Kanehiro Fujiyoshi, Shinya Yamanaka, Yoshiaki Toyama, Hideyuki Okano
Organizer
Neruroscience2008
Place of Presentation
Washington DC(ポスター発表)
Year and Date
20081115-20081119
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[Presentation] Cell therapy for spinal cord injury by neural stem/progenitor cells derived from induced pluripotent stem cells2008
Author(s)
Osahiko Tsuji, Kyoko Miura, Masaya Nakamura, Narihito Nagoshi, Kazuya Kitamura, Kanehiro Fujiyoshi, Gentaro Kumagai, Masahiko Mukaino, Hiroyuki Katoh, Yohei Okada, Yoshiaki Toyama, Shinya Yamanaka, Hideyuki Okano
Organizer
日本神経科学会
Place of Presentation
東京(口頭発表)
Year and Date
20080709-20080711
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