Establishment of cell therapy for transplanting neural crest-derived stem cells into spinal cord injury model of rodent and mammalian

2009 Fiscal Year Final Research Report

• Project/Area Number: 20791054
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: NAGOSHI Narihiro Keio University, 医学部, 助教 (10383837)
• Project Period (FY): 2008 – 2009
• Keywords: 再生医療 / 脊髄損傷 / 細胞移植 / 神経堤幹細胞 / 霊長類

Research Abstract

Neural crest-derived stem cells (NCSCs) are isolated from adult skin and bone marrow, and they have the ability to differentiate into neural cells. Therefore, NCSCs are expected as cell sources for transplantation into spinal cord injury. In our study, we transplanted NCSCs into spinal cord injury model of mice. We observed the transplanted NCSCs surviving in the injured spinal cord, differentiating into neural cells. Moreover, the transplanted NCSCs had no tumorigenesis, suggesting that transplantation of NCSCs are safe. We also isolated and characterized NCSCs from adult marmoset or human skin.

Research Products

• [Journal Article] Neural crest-derived stem cells display a wide variety of characteristics (2009)
  • Author(s): Nagoshi N, Shibata S, Nakamura M, Matsuzaki Y, Toyama Y, Okano H.
  • Journal Title: Journal of Cellular Biochemistry 107 Pages: 1046-1052
  • Peer Reviewed
• [Journal Article] Prospective identification, isolation and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow (2009)
  • Author(s): Morikawa S, Mabuchi Y, Kubota Y, Nagai Y, Niibe K, Hiratsu E, Suzuki S, Miyauchi-Hara C, Nagoshi N, Sunabori T, Shimmura S, Miyawaki A, Nakagawa T, Suda T, Okano H, Matsuzaki Y.
  • Journal Title: Journal of Experimental Medicine 206 Pages: 2483-2496
  • Peer Reviewed
• [Journal Article] Development of mesenchymal stem cells partially originate from neural crest (2009)
  • Author(s): Morikawa S, Mabuchi Y, Niibe K, Suzuki S, Nagoshi N, Sunabori T, Shimmura S, Nagai Y, Nakagawa T, Okano H, Matsuzaki Y.
  • Journal Title: Biochemical and Biophysical Research Communications 379 Pages: 1114-1119
  • Peer Reviewed
• [Journal Article] ES cell-derived NS/PCs transplantation after spinal cord injury (2009)
  • Author(s): Kumagai G, Okada Y, Yamane J, Nagoshi N, Kitamura K, Mukaino M, Tsuji O, Fujiyoshi K, Katoh H, Okada S, Shibata S, Matsuzaki Y, Toh S, Toyama Y, Nakamura M, Okano H.
  • Journal Title: PLoS One 4 Pages: e7706
  • Peer Reviewed
• [Journal Article] Regenerative medicine for spinal cord injury: Current status and open issues (2009)
  • Author(s): Nakamura M, Nagoshi N, Fujiyoshi K, Kaneko S, Toyama Y, Okano H.
  • Journal Title: Inflammation and Regeneration 29 Pages: 198-203
  • Peer Reviewed
• [Journal Article] 損傷脊髄に対する幹細胞移植 (2009)
  • Author(s): 名越慈人、岡野栄之
  • Journal Title: 最新医学幹細胞研究の最近の進歩(後篇) 64 Pages: 1404-16
• [Presentation] 損傷脊髄における自己修復機構ー内在性シュワン細胞の寄与ー (2010)
  • Author(s): 名越慈人、芝田晋介、中村雅也、松崎有未、戸山芳昭、岡野栄之
  • Organizer: 第9回日本再生医療学会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-19
• [Presentation] Schwann cell plasticity after spinal cord injury shown by neural crest lineage tracing (2009)
  • Author(s): Nagoshi N, Nakamura M, Shibata S, Matsuzaki Y, Okano H, Toyama Y
  • Organizer: Cervical Spine Research Society 37th Annual Meeting
  • Place of Presentation: Salt Lake City, UT, USA
  • Year and Date: 2009-12-05
• [Presentation] 損傷脊髄に対する神経堤由来細胞の寄与 (2009)
  • Author(s): 名越慈人、芝田晋介、松崎有未、岡野栄之、戸山芳昭、中村雅也
  • Organizer: 第24回日本整形外科学会基礎学術集会
  • Place of Presentation: 神奈川
  • Year and Date: 2009-11-05
• [Presentation] 神経堤幹細胞の発生と多能性-中枢神経の再生を目指して- (2009)
  • Author(s): 名越慈人
  • Organizer: 第10回運動器科学研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-19