2009 Fiscal Year Final Research Report
Identification and analysis of human spermatogonial stem cells
| Project/Area Number |
20791127
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Toho University |
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Principal Investigator |
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| Project Period (FY) |
2008 – 2009
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| Keywords | 再生医学 / 細胞・組織 / 幹細胞 / 男性不妊 / 精子 |
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| Research Abstract |
Isolated cells from testicular tissues were maintained in serum-free medium, selected growth factors, and STO feeder layer for 1 week. We found that magnetic-activated cell sorting (MACS) was an efficient method to obtain Thy-1+ cells from testis cell suspensions. Enriched Thy-1+ cells were present among unretained cells. Thy-1+ cells were expressed human embryonic stem cell genes. Sixteen weeks after transplanting the Thy-1+ cells into nude mice, there was no evidence of teratoma.
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