2009 Fiscal Year Final Research Report
Elucidation of the molecular mechanism by which NR5A family induces ovarian differentiation
| Project/Area Number |
20791139
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Fukui |
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Principal Investigator |
MIZUTANI Tetsuya University of Fukui, 医学部, 講師 (90322734)
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| Project Period (FY) |
2008 – 2009
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| Keywords | SF-1 / LRH-1 / 卵巣 / 幹細胞 / 分化 / StAR / クロマチン |
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| Research Abstract |
We identified novel SF-1/LRH-1 binding sites in human StAR gene about 3,000 bp upstream from the transcriptional start site. We demonstrated by a luciferase reporter system that the upstream region was important for the activation of gene expression. Interestingly, nucleosome eviction has been found at those SF-1/LRH-1 binding sites (both promoter and enhancer region) by ChIP assay. 3C analysis revealed that DNA loop was formed between the promoter and the enhancer regions. These results indicate that the novel enhancer region participates in the human StAR gene activation by orchestrating chromatin loop architecture. Furthermore, we characterized transcription factor C/EBPβ which is identified as one of the components of SF-1 complex by biochemical approach. We elucidated C/EBPβ is also important for SF-1/LRH-1-induced ovarian differentiation as a these partner.
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[Journal Article] Peroxisome proliferator-activated receptor-gamma coactivator-1 alpha regulates progesterone production in ovarian granulosa cells with steroidogenic factor-1 and liver receptor homolog-1.2010
Author(s)
Yazawa, T., Inaoka, Y., Okada, R., Mizutani, T., Yamazaki, Y., Usami, Y., Kuribayashi, M., Orisaka, M., Umezawa, A., Miyamoto, K.
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Journal Title
Mol. Endocrinol. 24(3)
Pages: 485-496
Peer Reviewed
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[Journal Article] Liver Receptor Homolog-1 regulates the transcription of steroidogenic enzymes and induces the differentiation of mesenchymal stem cells into steroidogenic cells.2009
Author(s)
Yazawa, T., Inaoka, Y., Mizutani, T., Kuribayashi, M., Umezawa, A., Miyamoto, K.
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Journal Title
Endocrinology 150(8)
Pages: 3885-3893
Peer Reviewed
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[Journal Article] Regulation of P450 oxidoreductase by gonadotropins in rat ovary and its effect on estrogen production. Reprod.2008
Author(s)
Inaoka, Y., Yazawa, T., Mizutani, T., Kokame, K., Kangawa, K., Uesaka, M., Umezawa, A., Miyamoto, K.
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Journal Title
Biol. Endocrinol. 16:6(1)
Pages: 62
Peer Reviewed
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[Presentation] Identification of a novel enhancer region in the human steroidogenic acute regulatory protein (StAR) gene.2010
Author(s)
Mizutani, T., Yazawa, T., Uesaka, M., Inaoka, Y., Ju, Y., Okada, R., Matsuura, K., Kamiki, Y., Umezawa, A., Miyamoto, K.
Organizer
14th International Congress of Endocrinology
Place of Presentation
Kyoto
Year and Date
20100327-20100330
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[Presentation] 2,3,7,8-tetrachlorodibenzo-p-dioxinのヒト羊膜上皮細胞におけるmatrix metalloproteinase-9発現誘導作用.2009
Author(s)
安部由美子, 小松原麻紀, 齋藤恵美, 戸田麻友穂, 水流奈己, 丸川りさ, 水谷哲也, 宮本薫
Organizer
日本下垂体研究会第24回学術集会
Place of Presentation
青森
Year and Date
20090827-20090829
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[Presentation] 羊膜上皮細胞におけるダイオキシンのmatrix metalloproteinase-9 (MMP-9)発現に対する作用2009
Author(s)
安部由美子, 小松原麻紀, 齋藤恵美, 戸田麻友穂, 平石光, 田村友宏, 笠原慶充, 定方久延, 勝俣祐介, 峯岸敬, 水谷哲也, 宮本薫
Organizer
第14回日本生殖内分泌学会学術集会
Place of Presentation
東京
Year and Date
2009-11-28
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