2010 Fiscal Year Final Research Report
Study for the differential regulation of pituitary gonadotropins
| Project/Area Number |
20791144
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Shimane University |
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Principal Investigator |
KANASAKI Haruhiko Shimane University, 医学部, 講師 (10325053)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ゴナドトロピン / 下垂体 / GnRH / 生殖内分泌 / PACAP |
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| Research Abstract |
Gene expression for PACAP and its own PAC1 receptor (PAC1R) were examined by pulsatile GnRH stimulation using perifusion system. In static culture, GnRH stimulated the expression of PACAP and PAC1R in gonadotroph cell line, LbT2. In pulsatile GnRH stimulation, low frequency of GnRH pulse preferentially increased PACAP and PAC1R expressions compared to that by low frequency GnRH pulse. PACAP increased both LH and FSH subunits dose-dependent manners. PACAP and PAC1R might be involved in GnRH pulse frequency-dependent gonadotropn subunit expressions.
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