2009 Fiscal Year Final Research Report
Proteomics to identify the targets of antiphospholipid antibodies in women with recurrent pregnancy loss
| Project/Area Number |
20791171
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
MINE Katsuya Nippon Medical School, 医学部, 助教 (60409216)
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| Project Period (FY) |
2008 – 2009
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| Keywords | プロテオミクス / 習慣流産 / 抗リン脂質抗体 / 不育症 |
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| Research Abstract |
Blood plasma samples were collected from women with recurrent pregnancy loss. Further, a clinical database of these patients was constructed.
Solid-phase plates comprising phospholipids embedded within cell membranes were constructed to extract phospholipids that bind to plasma proteins. The blood plasma samples were reacted with the plates to isolate the target plasma proteins. These proteins were visualized by 2-dimensional electrophoresis (2-DE). The protein spots on the 2-DE gels were identified by mass spectrometry.
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