2010 Fiscal Year Final Research Report
Localization and quantitative analysis of retinal protein by lasermicrodissection
| Project/Area Number |
20791252
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Shimane University |
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Principal Investigator |
KAIDZU Sachiko Shimane University, 医学部, 助教 (00325052)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 眼組織学 / 網膜光傷害 |
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| Research Abstract |
To study the change of antioxidants in retinal layers, we used lasemicrodissection. Light damaged rat retina were divided into 4 segments ; retinal pigment epithelium, rod outer segments～outer plexiform layer, inner nuclear layer～inner plexiform layer, ganglion cell layer～nerve fiber layer. Antioxidants were increased in each layer, particularly in rod outer segments～outer plexiform layer, and the change of localization and expression were almost similar to the results by immunohisochemical method. Lasermicrodissection is useful to study the localization and quantitative change of protein at cellular levels.
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