2010 Fiscal Year Final Research Report
Molecular-biological study for clarifying the etiology of lung hypoplasia of Gja1 knockout mice
| Project/Area Number |
20791300
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatric surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
NAGATA Kouji Kyushu University, 大学院・医学研究院・小児外科学分野, 助教 (20419568)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 小児呼吸器外科学 / Gja1 / connexin43 / 肺低形成 / 肺発達 |
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| Research Abstract |
Gja1 (Cx43) knockout mice die after birth due to hypoplastic lungs. Adult hetero Cx43 mice were mated. Newborn mice were divided into the wild group and the knockout group. H.E. staining in the knockout mice showed narrow airspaces and thicker interalveolar septae. The m-RNA expression of AQP-5, Sp-C and a-SMA was significantly lower in knockout mice than that in the wild mice. Immunohistochemical staining in all markers (AQP-5, Sp-C and a-SMA) showed the same result to the m-RNA expression.
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[Presentation] Cx43 plays important roles in lung development.2009
Author(s)
Kouji Nagata, Genshirou Esumi, Risa Teshiba, Kouji Masumoto, Keigo Yoshizaki, Satoshi Fukumoto, Kazuaki Nonaka, Tomoaki Taguchi.
Organizer
42nd Annual Meeting of Pacific Association of Pediatric Surgeons.
Place of Presentation
Hong Kong
Year and Date
2009-05-11
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