2009 Fiscal Year Final Research Report
Action potential-dependent ATP release from taste receptor cells : development of real-time micro determination for neurotransmitter release at single ceII level
| Project/Area Number |
20791355
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
MURATA Yoshihiro Kyushu University, 教育研究部・医療学系, 助教 (40377031)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 味細胞 / 活動電位 / シナプス / 神経伝達物質 |
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| Research Abstract |
Subsets of taste bud cells generate action potentials in response to sapid stimuli. Type II taste cells have many taste transduction molecules but lack well elaborated synapses, which leads to the question in the functional significance of action potentials in these cells. We examined the dependence of adenosine triphosphate (ATP) transmitter release from taste cells upon action potentials. To identify Type TT taste cells we used mice expressing a green fluorescence protein (GFP) transgene from the gustducin promoter. Action potentials were recorded by an electrode basolaterally attached to a. single GFP-positive taste cell. ATP release was monitored from gustducin-expressing taste cells by collecting the electrode solution immediately after tastant-stimulated action potentials and using a. luciferase assay to quantify ATP. Stimulation of gustducin-expressing taste cells with a. sweet, bitter, or umami compound on the apical membrane increased ATP levels in the electrode solution; the amount of ATP depended on the firing rate. Increased spontaneous firing rates also induced ATP release from gustducin-expressing taste cells. ATP release from gustducin-expressing taste cells was depressed by tetrodotoxin and inhibited below the detection limit by carbenoxolone. Our data support the hypothesis that action potentials in Type II taste cells enhance ATP release through hemichannels.
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Research Products
(31 results)
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[Journal Article] Discrimination of taste qualities among mouse fungi form taste bud cells2009
Author(s)
Yoshi da. R, Mi yauchi A, Y2 Suo T, kotaki M, Murata. Y, Yasumatsu K, Shi gemura N, Yanagawa Y, Obata K, Ueno H, Margolskee RF, Ninomi ya Y
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Journal Title
The Journal of Physiology Vol.587
Pages: 4425-4439
Peer Reviewed
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[Presentation] 甘味の受容・伝達・調節機構とATP2008
Author(s)
吉田竜介、村田芳博、安松啓子, 重村憲徳, 二ノ宮裕三
Organizer
生理研研究会『病態と細胞外プリン-治療標的としての可能性を探る」
Place of Presentation
自然科学研究機構岡崎コンファレンスセンター(愛知県岡崎市)
Year and Date
2008-09-05
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[Presentation] Temperature- and gurmarin-sensitivity of the chorda tympani nerve responses to sweetners in the wild-type, T1R3-, Ggust-, TRPM5-KO mice2008
Author(s)
Ohkuri T, Yasumatsu K, Jyotaki M, Kusuhara Y, Yasuo T, Murata Y, Margolskee RF, Ninomiya
Organizer
The 15th International Symposium on Olfaction and Taste
Place of Presentation
Hyatt Regency, San Francisco, USA
Year and Date
2008-07-25
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[Presentation] Modulation transmission of sweet taste information for energy homeostasis2008
Author(s)
Ninomiya Y, Yoshida. R, Murata. Y, Yasumatsu K, Ohkuri T, Shirosaki S, Sanematsu K, Yasuo T, Nakamura Y, Shigemura N
Organizer
The 15th International Symposium on Olfaction and Taste
Place of Presentation
Hyatt Regency, San Francisco, USA
Year and Date
2008-07-24
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