Physiological mechanisms of nicotinic receptors in nerve terminals terminated the salivary glands

2009 Fiscal Year Final Research Report

• Project/Area Number: 20791357
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: ONO Kentaro Kyushu Dental College, 歯学部, 助教 (40316154)
• Project Period (FY): 2008 – 2009
• Keywords: ニコチン / 唾液腺 / 細胞内カルシウム / 神経

Research Abstract

Although cigarette smoking induces salivary secretion, it is unclear the induction mechanism. In the present study, we investigated effects of nicotine on rat parotid gland cells using intracellular Ca^<2+> imaging. Nicotine increased intracellular Ca^<2+> concentration in salivary cells, and the response was blocked by drug-induced nerve synaptic blockage. Therefore, it is possible that nicotinic receptors in nerve terminals terminated the salivary gland mediate salivary secretion.

Research Products

• [Journal Article] Proteome analysis for rat saliva. (2009)
  • Author(s): Inenaga K, Yamada N, Yuji R, Kawai M, Uneyama H, Ono K, Suzuki E, Torii K.
  • Journal Title: The Journal of Medical Investigation 56 Pages: 224-227
• [Journal Article] Effects of pilocarpine and cevimeline on Ca^<2+> mobilization in rat parotid acini and ducts. (2009)
  • Author(s): Ono K, Inagaki T, Iida T, Hosokawa R, Inenaga K
  • Journal Title: The Journal of Medical Investigation 56 Pages: 375
• [Journal Article] Ca^<2+> mobilization by nicotine through synaptic activation in rat parotid acini. (2009)
  • Author(s): Iida T, Ono K, Inagaki T, Hosokawa R, Inenaga K.
  • Journal Title: The Journal of Medical Investigation 56 Pages: 376
• [Journal Article] Parotid salivary secretion induced by stimulation of periodontal regions with toothbrush in humans. (2009)
  • Author(s): Inenaga K, Inagaki T, Hosokawa R, Ono K.
  • Journal Title: The Journal of Medical Investigation. 56 Pages: 277
• [Journal Article] 味刺激による後味の強さと唾液分泌相関 (2009)
  • Author(s): 稲永清敏, 稲垣智浩, 小野堅太郎, 関根有紀, 河合美佐子, 畝山寿之, 鳥居邦夫
  • Journal Title: 16 Pages: 363-364
• [Journal Article] Small salivary gland size in patients with xerostomia of unknown etiology. (2009)
  • Author(s): Ono K, Tanaka T, Inoue H, Ansai T, Sato-Wakasugi N, Muraoka K, Yokota M, Takehara N, Morimoto Y, Inenaga K
  • Journal Title: Arch Oral Biol 54 Pages: 369-373
  • Peer Reviewed
• [Journal Article] Cell subpopulations of nicotine-sensitive subfornical organ neurons in rat. (2008)
  • Author(s): Ono K, Miyahara N, Inenaga K.
  • Journal Title: Neurosci Lett 442 Pages: 74-76
  • Peer Reviewed
• [Journal Article] Dynamic magnetic resonance sialography for patients with xerostomia. (2008)
  • Author(s): Tanaka T, Ono K, Ansai T, Yoshioka I, Habu M, Tomoyose T, Yamashita Y, Nishida I, Oda M, Kuroiwa H, Wakasugi-Sato N, Okabe S, Kito S, Takahashi T, Tominaga K, Inenaga K, Morimoto Y.
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod 106 Pages: 115-123
  • Peer Reviewed
• [Journal Article] Nicotinic receptor subtypes in rat subfornical organ neurons and glial cells. (2008)
  • Author(s): Ono K, Toyono T, Inenaga K.
  • Journal Title: Neuroscience 154 Pages: 994-1001
  • Peer Reviewed
• [Journal Article] Effect of central nicotinic activation on drinking behavior. (2008)
  • Author(s): Ono K, Hirase M, Kai A, Inenaga K.
  • Journal Title: Neuroreport 19 Pages: 845-849
  • Peer Reviewed
• [Journal Article] Intraperitoneal injection of pilocarpine activates neurons in the circumventricular organs and hypothalamus in rats. (2008)
  • Author(s): Inenaga K, Wakasugi-Sato N, Ono K, Hirase M, Honda E.
  • Journal Title: Brain Res 1200 Pages: 51-57
  • Peer Reviewed
• [Journal Article] Rheological properties of human saliva and salivary mucins. (2008)
  • Author(s): Inoue H, Ono K, Masuda W, Inagaki T, Yokota M, Inenaga K.
  • Journal Title: J Oral Biosci 50 Pages: 134-141
  • Peer Reviewed
• [Journal Article] Differences in the Ca^<2+> response resulting from neurotransmitter stimulations of rat parotid acini and ducts.
  • Author(s): Inagaki T, Ono K, Masuda W, Iida T, Hosokawa R, Inenaga K.
  • Journal Title: Auton Neurosci (in press)
  • Peer Reviewed
• [Presentation] 唾液プロテオーム解析. (2009)
  • Author(s): 稲永清敏, 山田尚之, 湯地玲子, 小野堅太郎, 河合美佐子, 畝山寿之, 鈴木榮一郎
  • Organizer: 第51回歯科基礎医学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-10
• [Presentation] ブラッシングによる耳下腺唾液分泌促進. (2009)
  • Author(s): 稲永清敏, 稲垣智浩, 小野堅太郎
  • Organizer: 第51回歯科基礎医学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-10
• [Presentation] Effects of pilocarpine and cevimeline on Ca^<2+> mobilization in rat parotid acini and ducts. (2009)
  • Author(s): Ono K, Inagaki T, Iida T, Hosokawa R, Inenaga K.
  • Organizer: The 11^<th> International symposium on exocrine secretion.
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 2009-07-25
• [Presentation] Ca^<2+> mobilization by nicotine through synaptic activation in rat parotid acini. (2009)
  • Author(s): Iida T, Ono K, Inagaki T, Iida T, Hosokawa R, Inenaga K.
  • Organizer: The 11^<th> International symposium on exocrine secretion.
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 2009-07-25
• [Presentation] Parotid salivary secretion induced by stimulation of periodontal regions with toothbrush in humans. (2009)
  • Author(s): Inenaga K, Inagaki T, Ono K.
  • Organizer: The 11^<th> International symposium on exocrine secretion
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 2009-07-24
• [Presentation] ラット耳下腺細胞における催唾剤による細胞内カルシウム濃度上昇. (2009)
  • Author(s): 小野堅太郎, 稲垣智浩, 細川隆司, 稲永清敏
  • Organizer: 第69回九州歯科学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-05-30
• [Presentation] Mechanism of drinking behavior by central activation of nicotinic receptor in rat. (2009)
  • Author(s): Inenaga K, Ono K, Miyahara N.
  • Organizer: The 8^<th> Korea-Japan joint symposium on Physiology
  • Place of Presentation: Pusan, Korea
  • Year and Date: 2009-01-16
• [Presentation] Nicotinic receptors in rat subfornical organ neurons and glial cells. (2008)
  • Author(s): Ono K, Toyono T, Inenaga K.
  • Organizer: Annual Meeting Society for Neuroscience
  • Place of Presentation: Washington D.C., USA
  • Year and Date: 2008-11-18
• [Presentation] ラット耳下腺細胞に対するピロカルピンおよびセビメリンの作用. (2008)
  • Author(s): 稲垣智浩, 小野堅太郎, 細川隆司, 稲永清敏
  • Organizer: 第50回歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-23
• [Presentation] 原因不明口腔乾燥症患者の唾液腺体積. (2008)
  • Author(s): 小野堅太郎, 稲永清敏
  • Organizer: 第50回歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-23
• [Presentation] 脳弓下器官におけるニコチン受容体と口腔機能 (2008)
  • Author(s): 小野堅太郎, 平瀬正輝, 稲垣智弘, 宮原宣高, 稲永清敏
  • Organizer: 第50回歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-23
• [Presentation] Different sensitivities to cholinergic and adrenergic stimulations in rat parotid. (2008)
  • Author(s): Inagaki T, Ono K, Hosokawa R, Inenaga K.
  • Organizer: Annual Meeting of International Association for Dental Research
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-10
• [Presentation] ラット耳下腺細胞のコリン性・アドレナリン性刺激による多様な反応 (2008)
  • Author(s): 稲垣智浩, 小野堅太郎, 増田渉, 細川隆司, 稲永清敏
  • Organizer: 第68回九州歯科学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-31
• [Book] 今日からはじめる!口腔乾燥症の臨床この主訴にこのアプローチ (2008)
  • Author(s): 安細敏弘、柿木保明(編著)
  • Total Pages: 6
  • Publisher: 医歯薬出版