2009 Fiscal Year Final Research Report

Identification and analysis in inducing factors of associated IkB kinase-alpha

Research Project

Project/Area Number	20791364
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Functional basic dentistry
Research Institution	The Nippon Dental University
Principal Investigator	MAEDA Genta The Nippon Dental University, 生命歯学部, 助教 (80434140)
Project Period (FY)	2008 – 2009
Keywords	IKKα / MALT1 / NFκB / メチレーション / エピジェネティック / GATA3
Research Abstract	Mucosa-associated lymphoid tissue1 (MALT1) activates nuclear factor -kB (NFκB) in lymphocyte lineages and have an effect on differentiation of epithelium. However, its expression and role in the pathology of malignant tumors of epithelial origin is not known. I examined MALT1 expression and its implications for the pathology. In this result, MALT1 expression is epigenetically inactivated during tumor progression, suggesting that the detection of MALT1 expression is a useful predictive and prognostic determinant in the clinical management. Determining binding sites of transcription factor is important for understanding the transcriptional control of target genes. I developed concatenate ChIP that rapidly and cost effectively specifies genomic fragments. Subsequently I investigated GATA3 binding sites and target genes to employed this new method. These data demonstrate that GATA3 binds to regulatory elements and controls target gene expression through physical interaction with core promoter regions.