2009 Fiscal Year Final Research Report
Observation of ultra-structures in human enamel and dentin by Ultra-high Voltage Electron Tomography
| Project/Area Number |
20791384
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
JIRO Miura Osaka University, 歯学部附属病院, 助教 (70437383)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 石灰化硬組織 / 電子線トモグラフィー / ナノテクノロジー |
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| Research Abstract |
Human enamel and dentin are hard and brittle mineralized tissues. It is difficult to prepare thin specimens (under 200 nm) of these tissues for transmission electron microscope observation without demineralizing them. We present a novel method for creating three-dimensional ultra structural images of human enamel and dentin using Focus Ion Beam method and ultra-high voltage electron microscope tomography.
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