2009 Fiscal Year Final Research Report
Gene analysis on the osteoblast proliferation and differentiation by culturing with biomaterial derived from natural resources.
| Project/Area Number |
20791389
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
YAMADA Shizuka Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (00363458)
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| Project Period (FY) |
2008 – 2009
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| Keywords | フィッシュコラーゲン / 骨芽細胞 / リアルタイムPCR法 / ウェスタンブロット分析 / von Kossa法 |
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| Research Abstract |
A human osteoblast was cultured with α-MEM containing fish collagen (FC) that was total concentration of 0.1%(w/v). Osteoblast proliferation by cell count and the expression of mineralization-related genes and proteins with real-time PCR and western blot analyses were investigated. Furthermore, von Kossa staining was performed to study the effect of FC on the osteoblast at the prolonged culture. The results revealed that cell proliferation and mineralization-related genes and proteins increased in the FC group compared with control group. After the incubation of prolonged period, mineralized nodule of FC group augmented than that of control group.
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