2009 Fiscal Year Final Research Report
A role of EZH2 in the human oral cancer and examination of the antitumor effect by EZH2siRNA
| Project/Area Number |
20791512
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Tottori University |
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Principal Investigator |
KODANI Kazunori Tottori University, 医学部, 助教 (60403391)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 口腔癌 / SCC / EZH2 / アテロコラーゲン / siRNA |
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| Research Abstract |
We studied siRNA of EZH2 in tumor cells and, for confirmation of the antitumor effect, designed EZH2siRNA five kinds. The cancer cell used HSC-3,SCC-KN,HSC-4 and used Ateloarray○R (KOKEN) for cell culture.
As for sequence (GTGTATGAGTTTAGAGTCA) (#4) of EZH2 siRNA, mRNA inhibition and cell proliferation inhibition showed depression effect as compared with other sequence together. # 4 inhibits mRNA manifestation in high efficiency.
The reduction effect of the cancer was found when we injected it into the nude mouse which we mixed EZH2SiRNA with atelocollagen, and transplanted a cancer cell.
As a result, as for EZH2siRNA, antitumor effect was found in the oral cancer.
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