2009 Fiscal Year Final Research Report
Developomental study of molecular target therapy to oral cancer with FGFBP.
| Project/Area Number |
20791519
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
SHINTANI Tomoaki Hiroshima University, 病院, 助教 (90403518)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 分子標的治療 / FGF結合蛋白 |
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| Research Abstract |
HBp17 siRNA was synthesized and transfected. Control scrambled siRNA was used. Quantitative RT-PCR analysis was performed. Then, we performed westernblotting analysis to compare with HBp17 in cell and culture media at 48hrs after transfection. Finaly growth assay was done. In HBp17-transfected cells, HBp17 mRNA expression was significantly decreased compared with control. HBp17 protein expression was also decreased in cell or culture media. In roliferation assay, growth of HBp17 siRNA trasfected-OSCC KA, UE and NA was inhibited.
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