2010 Fiscal Year Final Research Report
Basic research of molecular targeting therapy targeted to SP cells in oral cancer
| Project/Area Number |
20791534
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
YANAMOTO Souichi Nagasaki University, 病院, 講師 (10315260)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 口腔癌 / 癌幹細胞 / SP細胞 |
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| Research Abstract |
To determine whether human oral cancer cell lines contain a SP cell fraction, we performed flow cytometry analysis and sorting, followed by culturing in serum-free medium (SFM) using the SCC25 tongue cancer cell line. SCC25 cells contained 0.23% SP cells. The fraction of SP cells was available to grow in SFM cultures. SP cells showed higher mRNA expression of stem cell markers (ABCG2, Oct-4 and EpCAM) as compared with non-SP cells. Moreover, SP cells demonstrated more drug resistance to 5-FU, as compared with non-SP cells. The clone formation efficiency of SP cells was significantly higher than non-SP cells at an equal cell number.
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