2009 Fiscal Year Final Research Report
Three-dimensional quantification of blood/lymph vessel formation and inVaSion in oral cancers
| Project/Area Number |
20791553
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
YOSHIHITO Shimazu The Nippon Dental University, 生命歯学部, 講師 (10297947)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 口腔癌 / 脈管新生 / 脈管侵襲 / 免疫組織化学 / 3次元構築 |
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| Research Abstract |
The present study aimed at gaining 3D quantitative information about invasion front pattern, angiogenesis, and lymphangiogenesis in oral squamous cell carcinoma. During the approved 2-year-period, the P.I. developed the experimental protocol and procedures of histology-based 3D reconstruction comprising multiple immunolabeling of cancer constituents (e.g., carcinoma cells and blood/lymph endothelial cells), alignment and superposition of digitized images, and computer-assisted color segmentation of labeled targets. The reconstructed 3D images allow us to inspect visually and quantitatively the complex architecture of cancer lesions.
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