2009 Fiscal Year Final Research Report
Analysis of the mechanism of Calcium-sensing receptor activation in cementoblasts
| Project/Area Number |
20791605
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Periodontal dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
KANAYA Sousuke Tohoku University, 病院, 医員 (80375097)
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| Project Period (FY) |
2008 – 2009
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| Keywords | セメント芽細胞 / 細胞外カルシウム / FGF-2 |
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| Research Abstract |
We found that elevated levels of extracellular Ca^<2+> increase fibroblast growth factor (FGF)-2 gene expression in cementoblasts. Pretreatment with a protein kinase A (PKA) inhibitor, H89, or an adenylate cyclase inhibitor, MDL-12330A, inhibited Ca^<2+>-stimulated Fgf-2 expression. In contrast, pretreatment with the protein kinase C (PKC) inhibitor GF-109203X or the phospholipase C (PLC) inhibitor U73122 did not affect the expression of Fgf-2 transcripts, suggesting that the increase in Fgf-2 expression was dependent on the PKA but not the PLC/PKC signaling pathway.
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