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2010 Fiscal Year Final Research Report

Clarification of the acceleration mechanism of the production of oral malodor substances brought by the oral environmental factors and the interaction between various bacterial strains.

Research Project

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Project/Area Number 20791632
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Social dentistry
Research InstitutionTohoku University

Principal Investigator

WASHIO Jumpei  東北大学, 大学院・歯学研究科, 助教 (20400260)

Project Period (FY) 2008 – 2010
Keywords口臭 / 硫化水素 / 細菌 / 代謝
Research Abstract

The hydrogen sulfide(H_2S) is the main source of oral malodor. The production activity and the production mechanism of H_2S by the oral indigenous bacteria, Veillonella species and Actinomyces species, were examined. It was shown that these bacterial species have H_2S production activity and the activity was influenced by the environmental factors such as pH and the lactate concentration. Moreover, the mechanisms were slightly different depending on the bacterial strain even within same belonging. There has actually been suggested that there is possibility of H_2S production activity being influenced by its coexistence with other saccharolytic bacteria, therefore it is necessary to understand the cause of oral malodor in relation to bacteria not only as a single bacteria but also as the entire oral cavity including the oral biofilm microorganism and the oral environmental factors.

  • Research Products

    (38 results)

All 2011 2010 2009 2008

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (30 results) Book (4 results)

  • [Journal Article] Challenge to Metabolomics of Oral Biofilm: From "What Are They?" to "What Are They Doing?"2010

    • Author(s)
      Washio J, Mayanagi G, Takahashi N
    • Journal Title

      Journal of Oral Biosciences

      Volume: 52(3) Pages: 225-232

    • Peer Reviewed
  • [Journal Article] Metabolomics of supragingival plaque and oral bacteria.2010

    • Author(s)
      Takahashi N, Washio J, Mayanagi G.
    • Journal Title

      Journal of Dental research

      Volume: 89(12) Pages: 1383-1388

    • Peer Reviewed
  • [Journal Article] Transient acid-impairment of growth ability of oral Streptococcus, Actinomyces,and Lactobacillus: a possible ecological determinant in dental plaque.2009

    • Author(s)
      Horiuchi M, Washio J, Mayanagi H, Takahashi N.
    • Journal Title

      Oral Microbiol Immunol.

      Volume: 24 Pages: 319-324

    • Peer Reviewed
  • [Journal Article] Effects of alpha-amylase and its inhibitors on acid production from cooked starch by oral streptococci.2009

    • Author(s)
      Aizawa S, Miyasawa-Hori H, Nakajo K, Washio J, Mayanagi H, Fukumoto S, Takahashi N.
    • Journal Title

      Caries Res.

      Volume: 43(1) Pages: 17-24

    • Peer Reviewed
  • [Presentation] Divalent cations enhance short-time fluoride exposure-induced inhibition on Streptococcal acidgenicity2011

    • Author(s)
      Hitomi Domon-Tawaraya, Jumpei Washio(6名中 3番目)
    • Organizer
      58th annual meeting of Japanese association for dental research
    • Place of Presentation
      Kitakyushu, Japan
    • Year and Date
      2011-11-20
  • [Presentation] Metabolome analysis of oral plaque biofilm using CE-TOFMS2011

    • Author(s)
      Jumpei Washio(筆頭)
    • Organizer
      The 4th International symposium for Interface Oral Health Science in Sendai
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      2011-03-08
  • [Presentation] A high-sensitative alamarBlue method for evaluating bacterial adhesion to biomaterials2011

    • Author(s)
      Yoko Sakuma, Jumpei Washio(5名中 2番目)
    • Organizer
      The 4th International symposium for Interface Oral Health Science in Sendai
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      2011-03-08
  • [Presentation] Divalent cations enhance short-time fluoride exposure-induced inhibition on acid production by oral streptococci2011

    • Author(s)
      Hitomi Domon-Tawaraya, Jumpei Washio(6名中 3番目)
    • Organizer
      The 4th International symposium for Interface Oral Health Science in Sendai
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      2011-03-08
  • [Presentation] Actinomyces acid production; Effects of bicarbonate and fluoride at neural and acid pH.2011

    • Author(s)
      Junko Kawashima, Jumpei Washio(5名中 3番目)
    • Organizer
      The 4th International symposium for Interface Oral Health Science in Sendai
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      2011-03-08
  • [Presentation] Evaluation of pH at the interface between bacteria and restorative materials.2011

    • Author(s)
      Gen Mayanagi, Jumpei Washio(5名中 3番目)
    • Organizer
      The 4th International symposium for Interface Oral Health Science in Sendai
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      2011-03-08
  • [Presentation] Breath aceton in type 1 and type 2 diabetes mellitus.2011

    • Author(s)
      Naoko tanda, Jumpei Washio(5名中 2番目)
    • Organizer
      The 4th International symposium for Interface Oral Health Science in Sendai
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      2011-03-08
  • [Presentation] プラークバイオフィルムのメタボローム解析~糖代謝からアミノ酸代謝まで~2010

    • Author(s)
      鷲尾 純平(筆頭)
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      船堀
    • Year and Date
      2010-09-22
  • [Presentation] 短時間フッ化物曝露によStreptococcus mutans酸産生抑制効果に与える二価金属イオンの影響2010

    • Author(s)
      俵谷ひと美, 鷲尾 純平(6名中 3番目)
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      船堀
    • Year and Date
      2010-09-21
  • [Presentation] Evaluation of pH using an ISFET at the parasite-biomaterial interface.2010

    • Author(s)
      Gen Mayanagi, Jumpei Washio(4名中 2番目)
    • Organizer
      The 5th International Workshop on Nano, Bio and Amorphus Materials
    • Place of Presentation
      Miyagi, Japan
    • Year and Date
      2010-08-10
  • [Presentation] Metabolome analysis of glucose fermentation by dental plaque using CE-TOFMS.2010

    • Author(s)
      Jumpei Washio(筆頭)
    • Organizer
      88th general session and Exhibition of the International Association of Dental Research
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2010-07-16
  • [Presentation] An inproved alamarBlue Method for evaluating bacterial adhesion.2010

    • Author(s)
      Yoko Sakuma, Jumpei Washio(5名中 2番目)
    • Organizer
      88th general session and Exhibition of the International Association of Dental Research
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2010-07-16
  • [Presentation] Aceton in Mouth Air in type 2 Diabetes Mellitus.2010

    • Author(s)
      Naoko Tanda, Jumpei Washio(5名中 4番目)
    • Organizer
      88th general session and Exhibition of the International Association of Dental Research
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      2010-07-16
  • [Presentation] ISFETによるバイオフィルム-マテリアルインターフェイス環境測定2010

    • Author(s)
      真柳 弦, 鷲尾 純平(5名中 3番目)
    • Organizer
      第5回高機能インターフェイス科学カンファレンス
    • Place of Presentation
      仙台
    • Year and Date
      2010-03-14
  • [Presentation] う蝕関連口腔細菌の糖代謝メタボローム解析:糖代謝機能の類似と相違2009

    • Author(s)
      鷲尾 純平(筆頭)
    • Organizer
      第82回日本生化学大会
    • Place of Presentation
      神戸
    • Year and Date
      20091022-24
  • [Presentation] Hydrogen-sulfide production from various substrates by oral Veillonella and effects of lactate on the production.2009

    • Author(s)
      Jumpei Washio(筆頭)
    • Organizer
      The 3 rd International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      20090115-16
  • [Presentation] New quantitative fluorometry for evaluating oral bacteria adhesion to biomaterials.2009

    • Author(s)
      Sakuma Yoko, Washio Jumpei(5名中 2番目)
    • Organizer
      The 3 rd International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      20090115-16
  • [Presentation] Short-term effect of fluoride on acid production by Streptococcus mutans.2009

    • Author(s)
      Domon Hitomi, Washio Jumpei (6名中 3番目)
    • Organizer
      The 3 rd International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      20090115-16
  • [Presentation] Difference between age generation of oral health examination in a rural town.2009

    • Author(s)
      Tanda Naoko, Washio Jumpei(10名中 3 番目)
    • Organizer
      The 3 rd International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      20090115-16
  • [Presentation] CE-TOFMS を用いたプラークバイオフィルムの糖代謝メタボローム解析2009

    • Author(s)
      鷲尾 純平(筆頭)
    • Organizer
      第58回日本口腔衛生学会
    • Place of Presentation
      岐阜
    • Year and Date
      2009-10-10
  • [Presentation] Quantitative fluorometry for evaluating oral bacterial adhesion to biomaterials surface2009

    • Author(s)
      Yoko Sakuma, Jumpei Washio(5名中 2番目)
    • Organizer
      第58回国際歯科学会日本部会(JADR)学術大会(第2回PAPF/第1回APF学術大会との併催)
    • Place of Presentation
      武漢, 中国
    • Year and Date
      2009-09-23
  • [Presentation] CE-TOFMSを用いたStreptococcus mutansおよびActinomyces naeslundiiの糖代謝メタボローム解析2009

    • Author(s)
      鷲尾 純平(筆頭)
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟
    • Year and Date
      2009-09-10
  • [Presentation] 短時間フッ化物曝露によるStreptococcus mutansのフッ素吸着及び酸産生能への影響2009

    • Author(s)
      土門 ひと美, 鷲尾 純平(6名中 3番目)
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟
    • Year and Date
      2009-09-10
  • [Presentation] Actinomycesの糖代謝フッ化物耐性とその重炭酸による影響2009

    • Author(s)
      川嶋 順子, 鷲尾 純平(5名中 3番目)
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟
    • Year and Date
      2009-09-10
  • [Presentation] 口腔バイオフィルムのメタボローム解析の試み~何がいるかから、何をしているかへ~2009

    • Author(s)
      鷲尾 純平(筆頭)
    • Organizer
      第51回歯科基礎医学会学術大会サテライトシンポジウム
    • Place of Presentation
      新潟
    • Year and Date
      2009-09-09
  • [Presentation] 口腔常在菌VeillonellaやActinomycesによるシステインおよびシステイン含有ペプチドからの口臭成分(硫化水素)産生と、それに対する環境pH、乳酸の影響について2009

    • Author(s)
      鷲尾 純平(筆頭)
    • Organizer
      第6回東北大学バイオサイエンスシンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2009-06-16
  • [Presentation] Alamar blue[○!R]を用いたバイオマテリアル表面細菌付着量の新たな評価法の確立2009

    • Author(s)
      佐久間 陽子, 鷲尾 純平(5名中 2番目)
    • Organizer
      第6回東北大学バイオサイエンスシンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2009-06-16
  • [Presentation] 口腔Veillonella はcysteine含有ペプチドから硫化水素を産生し、乳酸はその硫化水素産生を促進する。2008

    • Author(s)
      鷲尾 純平(筆頭)
    • Organizer
      第50回歯科基礎医学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2008-09-25
  • [Presentation] バイオマテリアル表面への各種口腔細菌付着量の評価法2008

    • Author(s)
      佐久間陽子, 鷲尾 純平(5名中 2番目)
    • Organizer
      第50回歯科基礎医学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2008-09-25
  • [Presentation] Lactate enhances the production of hydrogen sulfide by oral Veillonella.2008

    • Author(s)
      Jumpei Washio(筆頭)
    • Organizer
      86th general session and Exhibition of the International Association of Dental Research
    • Place of Presentation
      Torronto, Canada
    • Year and Date
      2008-07-05
  • [Book] Hydrogen-sulfide production from various substrates by oral Veillonella and effects of lactate on the production. In: T. Sasano and O. Suzuki (eds.) Interface Oral Health Science 20092010

    • Author(s)
      Washio J (筆頭)
    • Total Pages
      250-251
    • Publisher
      Springer, New York
  • [Book] New quantitative fluorometry for evaluating oral bacteria adhesion to biomaterials. In: T. Sasano and O. Suzuki (eds.) Interface Oral Health Science 20092010

    • Author(s)
      Sakuma Y, Washio J (5名中 2番目)
    • Total Pages
      215-216
    • Publisher
      Springer, New York
  • [Book] Short-term effect of fluoride on acid production by Streptococcus mutans. In: T. Sasano and O. Suzuki (eds.) Interface Oral Health Science 20092010

    • Author(s)
      Domon H, Washio J (6名中 3番目)
    • Total Pages
      269-270
    • Publisher
      Springer, New York
  • [Book] Difference between age generation of oral health examination in a rural town. In: T. Sasano and O. Suzuki (eds.) Interface Oral Health Science 20092010

    • Author(s)
      Tanda N, Washio J (10名中 3番目)
    • Total Pages
      323-324
    • Publisher
      Springer, New York

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Published: 2013-07-31  

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